Bacterial Cell Wall Homeostasis Methods and Protocols
This volume brings together the most widely used and important protocols currently being employed in researching and understanding bacterial cell wall homeostasis. Chapters in Bacterial Cell Wall Homeostasis cover a variety of subjects, such as: modern mi
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Hee-Jeon Hong Editor
Bacterial Cell Wall Homeostasis Methods and Protocols
METHODS
IN
MOLECULAR BIOLOGY
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
Bacterial Cell Wall Homeostasis Methods and Protocols
Edited by
Hee-Jeon Hong Department of Biological and Medical Sciences, Faculty of Helath and Life Sciences, Oxford Brookes University, Oxford, UK; Department of Biochemistry, University of Cambridge, Cambridge, UK
Editor Hee-Jeon Hong Department of Biological and Medical Sciences Faculty of Health and Life Sciences Oxford Brookes University Oxford, UK Department of Biochemistry University of Cambridge Cambridge, UK
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-3674-8 ISBN 978-1-4939-3676-2 (eBook) DOI 10.1007/978-1-4939-3676-2 Library of Congress Control Number: 2016939971 © Springer Science+Business Media New York 2016 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. Printed on acid-free paper This Humana Press imprint is published by Springer Nature The registered company is Springer Science+Business Media LLC New York
Preface We live in a bacterial world. Bacteria have inhabited this planet for a few billion years longer than humans, and they have made themselves at home in every corner of it. From oceans to deserts, from frozen glaciers to hydrothermal vents, few environments are apparently too hostile for them to occupy and exploit. This of course also includes our own body tissues, and those of the animals and plants we rely on for our nutrition. They are even, unless especially treated, in the water that we drink. We have never known a world without them, but for most of our history we were completely unaware of their existence. Only 350 years ago—after nearly 200,000 years of evolution in their presence—did human technology advance to produce an optical microscope and ena
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