The Bacterial Flagellum Methods and Protocols
This volume examines the structure and dynamics of the bacterial flagellum using bacterial genetics, molecular biology, biochemistry, structural biology, biophysics, cell biology, and molecular dynamics simulation. The chapters are divided into 4 parts: P
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Tohru Minamino Keiichi Namba Editors
The Bacterial Flagellum Methods and Protocols
Methods
in
Molecular Biology
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
The Bacterial Flagellum Methods and Protocols
Edited by
Tohru Minamino Graduate School of Frontier Biosciences, Osaka University, Suita, Osaka, Japan
Keiichi Namba Graduate School of Frontier Biosciences, Osaka Univeristy, Suita, Osaka, Japan Quantitative Biology Center RIKEN, Suita, Osaka, Japan
Editors Tohru Minamino Graduate School of Frontier Biosciences Osaka University Suita, Osaka, Japan
Keiichi Namba Graduate School of Frontier Biosciences Osaka Univeristy Suita, Osaka, Japan Quantitative Biology Center RIKEN Suita, Osaka, Japan
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-6926-5 ISBN 978-1-4939-6927-2 (eBook) DOI 10.1007/978-1-4939-6927-2 Library of Congress Control Number: 2017935368 © Springer Science+Business Media LLC 2017 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. The publisher remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. Printed on acid-free paper This Humana Press imprint is published by Springer Nature The registered company is Springer Science+Business Media LLC The registered company address is: 233 Spring Street, New York, NY 10013, U.S.A.
Preface Many motile bacteria can swim in liquid environments and move on semi-solid surface by rotating flagella. The bacterial flagellum is a supramolecular assembly composed of 30 different proteins and consists of at least three parts: the basal body, the hook, and the filament. The basal body is embedded within the cell membranes and acts as a bidirectional rotary motor. The energy for motor rotation is supplied by cation influx driven by an electrochemical potential difference of specific ions
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