Biogas Production and Microbial Communities in the Anaerobic Digestion of Sewage Sludge Under Hydrothermal Pretreatment
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Biogas Production and Microbial Communities in the Anaerobic Digestion of Sewage Sludge Under Hydrothermal Pretreatment with Air and a Catalyst Van Toi Pham 1 & Pei-Hsun Wu 1 & Chung-Yu Guan 2 & Chia-Chi Chang 1 & Bo-Liang Liu 1 & Ching-Yuan Chang 1 & Chang-Ping Yu 1,3 Received: 20 May 2020 / Accepted: 30 September 2020 # Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract Hydrothermal pretreatment (HTP) of sewage sludge (SS) has been shown to improve the subsequent biogas production by anaerobic digestion (AD), but the effect of catalysts on HTP performance was less explored. This study intended to investigate the SS pretreatment by wet air oxidation (WAO) with the addition of K2CO3 as a catalyst on the performance of methane production by AD. WAO was found to improve the solubilization of SS, the soluble chemical oxygen demand, dissolved organic carbon, and total dissolved nitrogen. The methane yield from WAO increased from 202 mL/gVSin with no catalyst added to 277 mL/gVSin with 10 wt% of K2CO3 added at 180 °C with 30 min of residence time. Under this pretreatment condition, the highest methane production rate could achieve 15.8 mL/gVSin day, and the percentage of methane reached 73%. The structure of the microbial community involved in the AD was affected by the residence time, working gas, and catalyst of the HTP process. The results showed that Bacteroidetes, Bacteroidia, and SC103 were the dominant phylum, class, and genus of bacteria, respectively, of almost all of the samples. In addition, the most abundant archaeal order was Methanosarcinales, while Methanosaeta was the dominant archaeal genus of most of the samples. However, Methanosarcina largely increased the relative abundance, corresponding to the amount of K2CO3 catalyst used. The findings in this study demonstrated the potential use of K2CO3 during WAO of SS and implied the link between shift of methanogen community and the enhanced methane yield in AD. Keywords Methane production . Thermal hydrolysis . Wet air oxidation . K2CO3 catalyst . Microbial community
Abbreviations AD Anaerobic digestion AS Anaerobic sludge CH4 Methane COD Chemical oxygen demand CO2 Carbon dioxide DI De-ionized Electronic supplementary material The online version of this article (https://doi.org/10.1007/s12155-020-10199-4) contains supplementary material, which is available to authorized users. * Chang-Ping Yu [email protected] 1
Graduate Institute of Environmental Engineering, National Taiwan University, Taipei 106, Taiwan
2
Department of Environmental Engineering, National Ilan University, Yilan 260, Taiwan
3
Water Innovation, Low Carbon and Environmental Sustainability Research Center, National Taiwan University, Taipei 106, Taiwan
DGGE DOC FISH HTP N2 OTU PCR RT RT-PCR SCOD SS TDN TH TS VFAs VS VSR WAO WO
Denaturing gradient gel electrophoresis Dissolved organic carbon Fluorescent in situ hybridization Hydrothermal pretreatment Nitrogen Operational taxonomic units Polymerase chain reaction Reaction time Reverse transcription polymerase cha
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