Biological Applications of Synthesized ZnO Nanoparticles Using Pleurotus djamor Against Mosquito Larvicidal, Histopathol
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ORIGINAL PAPER
Biological Applications of Synthesized ZnO Nanoparticles Using Pleurotus djamor Against Mosquito Larvicidal, Histopathology, Antibacterial, Antioxidant and Anticancer Effect Kumar Manimaran1 • Govindasamy Balasubramani2 • Chinnasamy Ragavendran3 • Devarajan Natarajan3 Subban Murugesan1
•
Received: 3 June 2020 / Accepted: 26 October 2020 Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract The present study pertained to biosynthesis, characterization and biomedical application (larvicidal, histopathology, antibacterial, antioxidant and anticancer activity) of Zinc oxide nanoparticles (ZnONPs) from Pleurotus djamor. The synthesized NPs were characterized using spectral and microscopic analyses and further confirmed by UV–Visible spectrophotometer with apeak of 350 nm. The ZnONPs showed strong antioxidant property (DPPH, H2O2 and ABTS? radical assay) and expressed good larval toxicity against Ae. aegypti and Cx. quinquefasciatus (IVth instar larvae) with the least LC50 and LC90 values (10.1, 25.6 and 14.4, 31.7 mg/l) after 24 h treatment, respectively. We noticed the morphological changes (damaged anal papillae area and the cuticle layers) in the treated larvae. For the antibacterial assay, the highest growth inhibition zone was recorded in C. diphteriae (28.6 ± 0.3 mm), followed by P. fluorescens (27 ± 0.5 mm) and S. aureus (26.6 ± 1.5 mm). The in vitro cytotoxicity assay depicted a significant level of cytotoxic effects (LC50 values 42.26 lg/ml) of ZnONPs against the A549 lung cancer cells, even at low dose. The overall findings of the study suggest that P. djamor had the ability for the biosynthesis of ZnONPs and could act as an alternative biomedical agent for future therapeutic applications in medical avenues. Keywords ZnONPs Mosquito larvicidal Antibacterial Antioxidant A549 cell line
Introduction Nanotechnology is a new field for the biological synthesis of nanoparticles using molds, yeasts, and edible mushrooms [1]. In the present nanoparticle synthesis, an ecofriendly approach was utilized to avoid the toxicity from chemical reducing agents [2]. Several researchers have reported the eco-friendly nature of green synthesized & Subban Murugesan [email protected] 1
Edible and Medicinal Mushroom Laboratory, Department of Botany, School of Life Sciences, Periyar University, Periyar Palkalai Nagar, Salem, Tamil Nadu 636 011, India
2
School of Pharmaceutical Science and Technology, Health Science Platform, Tianjin University, Tianjin 300072, China
3
Natural Drug Research Laboratory, Department of Biotechnology, School of Biosciences, Periyar University, Tamil Nadu, Periyar Palkalai Nagar, Salem 636 011, India
nanoparticles using mushroom extract [3] and truffle [4]. Mostly, the higher amount of metallic nanoparticles was obtained, especially (edible mushroom, mycelia, and enzymes) from both the farm and laboratory mushroom [1]. However, few studies have successfully achieved for synthesizing ZnONPs [3], from the ed
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