Biologically synthesized of Au/Pt/ZnO nanoparticles using Arctium lappa extract and cytotoxic activity against leukemia
- PDF / 2,921,279 Bytes
- 11 Pages / 595.276 x 790.866 pts Page_size
- 98 Downloads / 235 Views
Biologically synthesized of Au/Pt/ZnO nanoparticles using Arctium lappa extract and cytotoxic activity against leukemia Renata Dobrucka 1 & Aleksandra Romaniuk-Drapała 2 & Mariusz Kaczmarek 3,4 Accepted: 22 September 2020 # The Author(s) 2020
Abstract The main objective of this work was to assess the cytotoxic activity of Au/Pt/ZnO nanoparticles synthesized using Arctium lappa extract against leukemia. The Au/Pt/ZnO nanoparticles obtained as a result of biological synthesis were characterized by UV-Vis, Scanning (SEM) and Transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FTIR), and Atomic Force Microscopy (AFM). The applied methods showed that the size of nanoparticles ranged from 10 to 40 nm. This work also assessed the cytotoxicity of Au/Pt/ZnO nanoparticles by means of MTT assay, and analyzed apoptosis as well as the influence of the cultivation time and concentration of Au/Pt/ZnO nanoparticles on the percentage of dead cells. The studies showed that the percentage of dead leukemia cells increased with the cultivation time and concentration of Au/Pt/ZnO nanoparticles. There was observed an increase in the percentage of cells in the G2/M phase, which suggests the stoppage of G2/M leading to cell death. The cytotoxicity of Au/Pt/ZnO nanoparticles determined by means of the MTT test indicated that the viability of leukemia cells practically disappeared when the concentration of the tested nanoparticles was 10 mol. Keywords Biological synthesis . Au/Pt/ZnO nanoparticles . Arctium lappa extract
1 Introduction Metal nanoparticles obtained from nanotechnology have gained global interest because of their broad applicability in biomedical and physiochemical fields. Monometallic nanoparticles are used for many different purposes (Nascimento et al. 2018). Biomedical application present nanoporous materials which take important role for drug delivery (Li et al. 2018a; Li et al. 2018b). Also, the control over the porous * Mariusz Kaczmarek [email protected] 1
Department of Non-Food Products Quality and Packaging Development, Institute of Quality Science, Poznań University of Economics and Business, al. Niepodległości 10, 61-875 Poznań, Poland
2
Department of Clinical Chemistry and Molecular Diagnostics, Poznan University of Medical Sciences, 49 Przybyszewskiego St, 60-355 Poznań, Poland
3
Department of Cancer Immunology, Chair of Medical Biotechnology, Poznan University of Medical Sciences, Garbary 15 Str, 61-866 Poznan, Poland
4
Gene Therapy Laboratory, Department of Cancer Diagnostics and Immunology, Greater Poland Cancer Centre, Garbary 15 Str, 61-866 Poznan, Poland
structures is fundamental to elucidate the relationship between the structures and material performance (Li et al. 2019a; Jiang et al. 2018). In the case of metal nanoparticles the interesting solutions are derived from the combination of metal nanoparticles. When combined, they tend to demonstrate new or stronger characteristics, different from the ones exhibited by their individual components (Zhang et al.
Data Loading...
