Modulation of Physico-Chemical and Biological Properties of Silver Nanoparticles Synthesized Using Aqueous Extract of Fl
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ORIGINAL PAPER
Modulation of Physico-Chemical and Biological Properties of Silver Nanoparticles Synthesized Using Aqueous Extract of Flamboyant (Delonix regia var. flavida, Fabaceae) Seeds Julia Moreira Pupe1,2
•
Luciano Paulino Silva1,2
Received: 16 April 2020 Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract Delonix regia has bioactive phytochemicals present in leaves, seeds, roots, and flowers. Considering that these compounds are common in extracts used for silver nanoparticles (AgNPs) biosynthesis, this study investigated the potential of D. regia seeds aqueous extract (DRE) in different concentrations to produce AgNPs (DRE-AgNPs). DRE composition was investigated by qualitative assays, and physico-chemical/structural characterization of AgNPs was performed by UV– Visible spectroscopy, dynamic light scattering (DLS), Zeta potential, and atomic force microscopy (AFM). Antibacterial effect of DRE-AgNPs was evaluated against Escherichia coli and Staphylococcus aureus. DRE presented mainly tannins and saponins. Additionally, the absorbance of each DRE-AgNPs was positively correlated to DRE concentration. At higher DRE concentrations, AgNPs were larger, regarding Z average; tended to monodispersity; and presented more negative Zeta potentials. Hydrodynamic diameters of DRE-AgNPs were measured by number distribution and only AgNPs synthesized with DRE 2 mg ml-1 (DRE-AgNPs 2) presented a single population of particles. Thus, DRE-AgNP 2 was analyzed by AFM and exhibited a spherical shape and an average height of 18.39 ± 1.59 nm. Most of DRE-AgNPs presented antibacterial effects against E. coli and S. aureus at 128 or 256 lmol l-1. In conclusion, different DRE concentrations modulated physico-chemical characteristics of AgNPs with antibacterial activity. Keywords AgNPs Green synthesis Plant extracts Tunable nanoparticles Antibacterial activity
Introduction Metallic nanoparticles (MNPs) are nanostructured metals that present different characteristics when compared to microstructured particles and typically have at least one dimension less than 100 nm [1]. These structures have gained much attention due to their optical, chemical, physical, and biological properties that make them useful in fields related to agriculture, pharmaceutical industry, electronics, bioremediation, and textile industry [2–6].
& Luciano Paulino Silva [email protected] 1
Embrapa Recursos Gene´ticos E Biotecnologia, PBI, Pq. Est. Biol. Final W5 Norte, Asa Norte, Brası´lia, DF 70770-917, Brazil
2
Instituto de Cieˆncias Biolo´gicas, Universidade de Brası´lia, Brası´lia, DF 70910-900, Brazil
Among MNPs, silver nanoparticles (AgNPs) have highlighted enhanced interest due to their various applications that include biosensors, antimicrobial agents, catalysts, and water purifiers [7]. The synthesis of AgNPs occurs by chemical, physical, or biological processes [8]. In contrast to chemical and physical methods, biological approaches do not require harmful a
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