Biological Compound Capping of Silver Nanoparticle with the Seed Extracts of Blackcumin ( Nigella sativa ): A Potential
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Biological Compound Capping of Silver Nanoparticle with the Seed Extracts of Blackcumin (Nigella sativa): A Potential Antibacterial, Antidiabetic, Anti‑inflammatory, and Antioxidant Sekar Vijayakumar1,2 · Mani Divya2 · Baskaralingam Vaseeharan2 · Jingdi Chen1 · Muniyandi Biruntha3 · Luciano Paulino Silva4 · Esteban F. Durán‑Lara5,6 · Krishnan Shreema7 · Shivendu Ranjan8 · Nandita Dasgupta8 Received: 21 June 2020 / Accepted: 13 August 2020 © Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract It is a well explored facts that need of biosynthesized nanomaterials has emerged for its several advantages in biomedical applications and the search for potential biosynthesized-nanomaterials is still ongoing. In this study an attempt was made to meet the requirement of potential biosynthesized-silver nanoparticles using the aqueous seed extract of N. sativa (Bc-AgNPs). X-ray powder diffraction (XRD) confirms the biosynthesis of silver nanoparticle (supports the observation of UV–Vis spectroscopy) with face centred cubic structure. Fourier-transform infrared spectroscopy (FTIR) confirms the capping of the active ingredients of seed extract and further the average size of 34 nm was confirmed by high-resolution transmission electron microscopy (HR-TEM) and energy dispersive X-ray analysis (EDX) confirms the purity of Bc-AgNPs. Due to the capping of the active ingredients of seed extract, the Bc-AgNPs have shown significant antibacterial activity against Gram negative bacteria and at the lower concentration of 100 µg*ml−1 it has 89% scavenging activity against free radicals. A strong anti-inflammatory (membrane stabilization) activity towards RBCs (98% at 100 µg*ml−1) was recorded when compared to the positive control (aceclofenac). In addition to the previous activities, a greater inhibition of carbohydrate hydrolyzing enzymes such as α-amylase, α-glucosidase and DPP-IV highlighted the antidiabetic potential of these green synthesized AgNPs. Overall, the efficient bioactivities of Bc-AgNPs advocates its future potential in biomedical products. Keywords Diabetes mellitus · Nigella sativa · Silver nanoparticles · Antioxidant · Anti-inflammatory
Electronic supplementary material The online version of this article (https://doi.org/10.1007/s10904-020-01713-4) contains supplementary material, which is available to authorized users. * Sekar Vijayakumar [email protected]; [email protected] Jingdi Chen [email protected] Luciano Paulino Silva [email protected]
Shivendu Ranjan [email protected]; [email protected]
1
Marine College, Shandong University, Weihai 264209, People’s Republic of China
Nanobiosciences and Nanopharmacology Division, Biomaterials and Biotechnology in Animal Health Lab, Department of Animal Health and Management, Alagappa University, Science campus 6th Floor, Karaikudi, Tamil Nadu 630004, India
2
Abbreviations AgNO3 Silver nitrate ATCC American Type Culture Collection BC Black cumin 3
Vermiculture Technology Lab, Department of Animal
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