Biogenically Synthesized Silver Nanoparticles Using Endophyte Fungal Extract of Ocimum tenuiflorum and Evaluation of Bio
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ORIGINAL PAPER
Biogenically Synthesized Silver Nanoparticles Using Endophyte Fungal Extract of Ocimum tenuiflorum and Evaluation of Biomedical Properties Hemashekhar Bagur1 • Chandrappa Chinna Poojari1 • Govindappa Melappa2 • Rajesh Rangappa3 N. Chandrasekhar1 • Prathap Somu4
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Received: 26 September 2019 Springer Science+Business Media, LLC, part of Springer Nature 2019
Abstract In the present work, AgNPs have been prepared using an extract of Exserohilum rostrata, an endophyte fungus isolated from Ocimum tenuiflorum leaf and characterized using TEM, SEM, DLS, XRD, FT-IR, etc. The FT-IR analysis confirmed the capping of AgNPs with bioactive molecules of endophyte extract, thereby importing addition or enhancing inherent AgNPs therapeutic properties. Both endophyte extract and AgNPs offered significant inhibition in Gram-positive and Gram-negative bacteria. Further, we also demonstrated its ability to inhibit bacterial biofilm formation of P. aeruginosa and S. aureus. Furthermore, we also demontrated excellent antioxidant and anti-inflammatory activity of both endophyte extract and AgNPs. Moreover, we observed ehanced antibacterial, anti-inflammatory, and antioxidant activity of AgNPs which is due to the synergistic effect of the bioactive agent forming the corona of AgNPs and with inhabitant activity of AgNPs. We also demonstrated the antimitotic activity in A. cepa and antiproliferative activity in breast cancer cells. AgNPs have also found to be excellent compatibility with healthy human keratinocyte cells and RBC cells. Hence, we might say that biogenically synthesized AgNPs using endophyte extract possess anti-bacterial, anti-inflammatory, and anti-oxidant ability as well as antiproliferative activity in breast cancer cells and thus possible found its application in the biomedical industry due to it’s eco-friendly and cost-effectiveness. Keywords Silver nanoparticles Endophyte fungi Green synthesis Biocompatibility Anti-oxidant activity
Introduction
Electronic supplementary material The online version of this article (https://doi.org/10.1007/s10876-019-01731-4) contains supplementary material, which is available to authorized users. & Chandrappa Chinna Poojari [email protected] & Prathap Somu [email protected] 1
Department of Biotechnology, Shridevi Institute of Engineering and Technology, Tumakuru, Karnataka 572106, India
2
Department of Botany, Davangere University, Davangere, Karnataka, India
3
Department of Biotechnology, Tumkur University, Tumakuru, Karnataka, India
4
Department of Biotechnology, GIET University, Gunupur, Odisha, India
Nanoparticles have gaining a great deal of importance due to the ability acquire to novel new and/or improvement in existing physicochemical properties when compared to their bulk form such as physical, chemical, optical, magnetic, thermal, dielectric, and biological properties [1]. Further nanoparticles have already a vast range of applications in the various field of science and engineering, i
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