Biomass, nutrient uptake and fatty acid composition of Chlamydomonas sp. ICE-L in response to different nitrogen sources
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Biomass, nutrient uptake and fatty acid composition of Chlamydomonas sp. ICE-L in response to different nitrogen sources AN Meiling1, 2†, WANG Yibin1, 3†, LIU Fangming1, 3, QI Xiaoqing1, ZHENG Zhou1, 3, YE Naihao4, SUN Chengjun1, MIAO Jinlai1, 2, 3* 1 The First Institute of Oceanography, State Oceanic Administration, Qingdao 266061, China 2 Qingdao University, Qingdao 266071, China 3 Qingdao National Laboratory for Marine Science and Technology, Qingdao 266235, China 4 Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China
Received 12 December 2015; accepted 14 April 2016 ©The Chinese Society of Oceanography and Springer-Verlag Berlin Heidelberg 2017
Abstract
Nitrogen removal from media by microalgae provides the potential benefit of producing lipids for biodiesel and biomass. However, research is limited on algal growth and biomass under different nitrogen sources and provides little insight in terms of biofuel production. We studied the influences of nitrogen sources on cell growth and lipid accumulation of Chlamydomonas sp. ICE-L, one of a promising oil rich micro algal species. Chlamydomonas sp. ICE-L grown in NH4Cl medium had maximum growth rate. While the highest dry biomass of 0.28 g/L was obtained in media containing NH4NO3, the highest lipid content of 0.21 g/g was achieved under nitrogendeficiency condition with a dry biomass of 0.24 g/L. In terms of total polyunsaturated fatty acids (PUFAs) production, NH 4 NO 3 and NH 4 Cl media performed better than nitrogen-deficiency and KNO 3 media. Furthermore, NH4NO3 and NH4Cl media elucidated better results on C18:3 and C20:5 productions while KNO3 and -N conditions were better in C16:0, C18:1 and C18:2, comparatively. Key words: Antarctic ice alga, Chlamydomonas sp. ICE-L, nitrogen-deficiency condition, nutrient uptake, polyunsaturated fatty acids (PUFAs) Citation: An Meiling, Wang Yibin, Liu Fangming, Qi Xiaoqing, Zheng Zhou, Ye Naihao, Sun Chengjun, Miao Jinlai. 2017. Biomass, nutrient uptake and fatty acid composition of Chlamydomonas sp. ICE-L in response to different nitrogen sources. Acta Oceanologica Sinica, 36(2): 105–110, doi: 10.1007/s13131-017-0984-4
1 Introduction Microalgae have received considerable attention as potential next-generation feed stocks for advanced biofuels, because microalgae biofuel is renewable, environmentally friendly, with wide applications (Scott et al., 2010; Wijffels and Barbosa, 2010). Specifically, compared to other energy crops, microalgae have advantages of faster growth, higher photosynthetic efficiency and tolerance to variable environmental conditions. Thousand species of microalgae, such as Nannochloropsis oceanica (Dong et al., 2013), Haematococcus pluvialis (Lei et al., 2012) and Isochrysis zhangjiangensis (Feng et al., 2011a), have been screened for candidates for biodiesel production (Hu et al., 2008). In Nannochloropsis, the fatty acids (FAs) content can accumulate up to 50% of dry weight with a considerable amount of eicosapentaen oic acid (EPA, C20:5) (Recht et al
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