Tumor Profiling Methods and Protocols

This book provides a practical guide to current methods for profiling and interpreting genomic alterations in tumors. Chapters detail methods to interrogate DNA variation, RNA expression, and epigenetic changes using both next-generation sequencing and mi

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Sarah S. Murray Editor

Tumor Profiling Methods and Protocols

METHODS

IN

MOLECULAR BIOLOGY

Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK

For further volumes: http://www.springer.com/series/7651

Tumor Profiling Methods and Protocols

Edited by

Sarah S. Murray Department of Pathology, University of California San Diego, La Jolla, CA, USA Center for Advanced Laboratory Medicine, University of California San Diego Health, La Jolla, CA, USA

Editor Sarah S. Murray Department of Pathology University of California San Diego La Jolla, CA, USA Center for Advanced Laboratory Medicine University of California San Diego Health La Jolla, CA, USA

ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-9002-3 ISBN 978-1-4939-9004-7 (eBook) https://doi.org/10.1007/978-1-4939-9004-7 Library of Congress Control Number: 2018965213 © Springer Science+Business Media, LLC, part of Springer Nature 2019 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors, and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. The publisher remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. This Humana Press imprint is published by the registered company Springer Science+Business Media, LLC, part of Springer Nature. The registered company address is: 233 Spring Street, New York, NY 10013, U.S.A.

Preface The aim of this book is to provide a practical guide to current methods for profiling and interpreting genomic alterations in tumors. This book will also provide a description of the main applications where tumor genome profiling is used in both research and clinical settings. A wide range of professionals from both basic science and medicine will find this guide to be a useful resource for learning about technical details, applications, and strengths and limitations of the latest technologies as applied to this increasingly important field. Researchers including molecular biologists, geneticists