Cell Fusion Overviews and Methods
This volume details protocols on detection and analysis of fusion events in various cell types in vitro and in vivo. Additional, protocols aim to facilitate the future highly relevant process of engineering cells for specific purposes, such as generation
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Kurt Pfannkuche Editor
Cell Fusion Overviews and Methods Second Edition
METHODS
IN
MOLECULAR BIOLOGY
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
Cell Fusion Overviews and Methods Second Edition
Edited by
Kurt Pfannkuche Center for Physiology and Pathophysiology, University of Cologne, Cologne, Germany
Editor Kurt Pfannkuche Center for Physiology and Pathophysiology University of Cologne Cologne, Germany
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-2702-9 ISBN 978-1-4939-2703-6 (eBook) DOI 10.1007/978-1-4939-2703-6 Library of Congress Control Number: 2015938703 Springer New York Heidelberg Dordrecht London © Springer Science+Business Media New York 2015 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. Printed on acid-free paper Humana Press is a brand of Springer Springer Science+Business Media LLC New York is part of Springer Science+Business Media (www.springer.com)
Preface The event of cell-cell fusion is involved in a variety of physiological, but importantly also of pathophysiological processes. As a prominent example, the formation of skeletal muscle fibers depends on the fusion of myoblast cells to form giant syncytia. This book addresses this fascinating process that takes place during muscle development and regeneration with several chapters highlighting methods to analyze cell fusion in skeletal muscle. Critically, cell fusion represents an indispensable mechanism in various processes of development and regeneration. Furthermore, the process of cell fusion may rejuvenate aging or damaged cells upon their fusion with certain stem or progenitor cells. These aspects are covered by a selection of protocols that address the fusion process in a variety of cell types beyond skeletal muscle tissue. High definition methods are presented to detect fusion events in vitro and in
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