3D Cell Culture Methods and Protocols
This book provides an overview of established 3D cell culture assays from leaders in the field. Their contributions cover a wide spectrum of techniques and approaches for 3D cell culture, from organoid cultures through organotypic models to microfluidic a
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Zuzana Koledova Editor
3D Cell Culture Methods and Protocols
Methods
in
Molecular Biology
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
3D Cell Culture Methods and Protocols
Edited by
Zuzana Koledova Department of Histology and Embryology, Masaryk University, Brno, Czech Republic
Editor Zuzana Koledova Department of Histology and Embryology Masaryk University Brno, Czech Republic
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-7019-3 ISBN 978-1-4939-7021-6 (eBook) DOI 10.1007/978-1-4939-7021-6 Library of Congress Control Number: 2017939584 © Springer Science+Business Media LLC 2017 Chapter 15 is distributed under the terms of the Creative Commons Attribution 4.0 International License (http:// creativecommons.org/licenses/by/4.0/). For further details see license information in the chapter. This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. The publisher remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. Printed on acid-free paper This Humana Press imprint is published by Springer Nature The registered company is Springer Science+Business Media LLC The registered company address is: 233 Spring Street, New York, NY 10013, U.S.A.
Preface In the last decade, there has been a boom in three-dimensional (3D) cell culture methods. This type of cell culture aims to completely recapitulate the 3D organization of cells and cell-cell and cell-matrix interactions in vitro to closely mimic a typical organ microarchitecture and microenvironmental signaling. Thereby, 3D cell cultures represent more accurate and physiologically more relevant models and thus bridge the gap between more traditional 2D cell cultures in Petri dish and live organism. They provide a malleable platform and a less costly and more ethical alternative to in vivo models
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