T-Cell Trafficking Methods and Protocols
This second edition provides updated and new chapters on T-Cell trafficking. In addition to detailed experimental procedures, the interested reader will find informative introductory chapters on the relevance of T-Cell trafficking in thymic population and
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George Edward Rainger Helen M. Mcgettrick Editors
T-Cell Trafficking Methods and Protocols Second Edition
Methods
in
Molecular Biology
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
T-Cell Trafficking Methods and Protocols Second Edition
Edited by
George Edward Rainger Institute of Cardiovascular Sciences, University of Birmingham, Birmingham, UK
Helen M. Mcgettrick Institute of Inflammation and Ageing, College of Medicine and Dental Sciences, University of Birmingham, Birmingham, UK
Editors George Edward Rainger Institute of Cardiovascular Sciences University of Birmingham Birmingham, UK
Helen M. Mcgettrick Institute of Inflammation and Ageing College of Medicine and Dental Sciences University of Birmingham Birmingham, UK
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-6929-6 ISBN 978-1-4939-6931-9 (eBook) DOI 10.1007/978-1-4939-6931-9 Library of Congress Control Number: 2017932550 © Springer Science+Business Media LLC 2017 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. The publisher remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. Printed on acid-free paper This Humana Press imprint is published by Springer Nature The registered company is Springer Science+Business Media LLC The registered company address is: 233 Spring Street, New York, NY 10013, U.S.A.
Preface Welcome to the second edition of Methods and Protocols for assessing T cell Trafficking, in the Methods in Molecular Biology series. The trafficking of T cells is relevant in numerous contexts. It occurs during population and maturation of T cells in the thymus and is required for dissemination of antigen naïve T cells to the secondary lymphatic organs where immune responses are initiated. Indeed, T cell trafficking within lymph nodes plays an important role
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