Characterization and genome sequencing of three Aeromonas hydrophila -specific phages, CF8, PS1, and PS2
- PDF / 978,190 Bytes
- 4 Pages / 595.276 x 790.866 pts Page_size
- 79 Downloads / 198 Views
ANNOTATED SEQUENCE RECORD
Characterization and genome sequencing of three Aeromonas hydrophila‑specific phages, CF8, PS1, and PS2 Sumeet Rai1 · Anuj Tyagi1 · Anu Kalia2 · B. T. Naveen Kumar1 · Prince Garg2 · Niraj K. Singh3 Received: 27 October 2019 / Accepted: 2 April 2020 © Springer-Verlag GmbH Austria, part of Springer Nature 2020
Abstract Aeromonas hydrophila is an important finfish pathogen, besides being an opportunistic human pathogen. In the present study, the genomes of three A. hydrophila-specific phages, CF8, PS1, and PS2, were isolated, characterized and sequenced. Transmission electron microscopy showed that all three phages had typical Myoviridae morphology. The linear dsDNA genomes of CF8, PS1, and PS2 were 238,150 bp, 237,367 bp, and 240,447 bp in length, with a GC content of 42.2%, 38.8%, and 38.8%, respectively. The low sequence similarity (67.6% - 69.8% identity with 27.0% - 29.0% query coverage) to other phage genomes in the NCBI database indicated the novel nature of the CF8, PS1, and PS2 genomes. A total of 244, 247, and 250 open reading frames (ORFs) were predicted in the CF8, PS1, and PS2 genome, respectively. During the annotation process, functional predictions were made for 28-31 ORFs, while the rest were classified as “hypothetical proteins” with yet unknown functions. Genes for tRNAs were also detected in all phage genomes. As all three phages in the present study had a very narrow host range with lytic activity against only one strain of A. hydrophila, these phages could be good candidates for phage typing applications. Moreover, the endolysin- and lytic-transglycosylase-encoding genes could be used for recombinant cloning and expression of anti-microbial proteins. Among the various bacterial pathogens, Aeromonas hydrophila is of specific concern in finfish aquaculture. It is a Gram-negative, facultatively anaerobic, motile, rod-shaped bacterium. A. hydrophila is associated with hemorrhagic septicemia and tail and fin rot in fishes, leading to secondary fungal infection and mortality in severe cases [1]. Although prophylactic measures are regularly taken in aquaculture, such as maintenance of optimum culture conditions and the Handling Editor: Johannes Wittmann. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00705-020-04644-0) contains supplementary material, which is available to authorized users. * Anuj Tyagi [email protected] 1
College of Fisheries, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab 141004, India
2
Electron Microscopy and Nanoscience Laboratory, Punjab Agricultural University, Ludhiana, Punjab 141004, India
3
School of Animal Biotechnology, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab 141004, India
use of probiotics and immunostimulants, the application of medicated feed containing antibiotics often becomes the method of choice for treatment of bacterial diseases. The recent emergence of antibiotic-resistant bacteria has led to renewed in
Data Loading...
