Characterization of Novel Bacteriophage AhyVDH1 and Its Lytic Activity Against Aeromonas hydrophila
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Characterization of Novel Bacteriophage AhyVDH1 and Its Lytic Activity Against Aeromonas hydrophila Yahui Cheng1 · Dongye Gao1 · Yunsheng Xia1 · Ziyi Wang1 · Meng Bai2 · Kaijun Luo3 · Xiaolong Cui2,3,4 · Yongxia Wang2,3 · Shiying Zhang1 · Wei Xiao2,3 Received: 4 May 2020 / Accepted: 28 October 2020 © Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract Phage therapy is an alternative approach to overcome the problem of multidrug-resistant bacteria. Here, a novel bacteriophage AhyVDH1, which infects Aeromonas hydrophila 4572, was isolated and its morphology, one-step growth curve, lytic activity, stability under various conditions, and genome were investigated. Transmission electron microscopy revealed that AhyVDH1 has an icosahedral head 49 nm in diameter and a contractile tail 127 nm in length, suggesting that it belongs to the family Myoviridae. AhyVDH1 showed strong adsorption to the surface of A. hydrophila 4572 (90% in 10 min). The latent period of AhyVDH1 was shown to be 50 min, and the burst size was 274 plaque-forming unit/infected cell. AhyVDH1 was stable at 30 °C for 1 h and lost infectivity after20 min of heating at 60 °C. Infectivity remained unaffected at pH 6–7 for 1 h, while the bacteriophage was inactivated at pH 11. AhyVDH1 has a 39,175-bp genome, with a 58% G + C content and 59 open reading frames. BLAST analysis indicated that the genome sequence of phage AhyVDH1 was related to that of Aeromonas phage Ahp2. Both time and MOI-dependent in vitro A. hydrophila growth inhibition were observed with AhyVDH1.Re-growth of the host bacteria appeared about 12 h after treatment, suggesting its potential therapeutic value in treating A. hydrophila infections, but phage cocktails should be developed.
Introduction
Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00284-020-02279-7) contains supplementary material, which is available to authorized users. * Shiying Zhang amy‑[email protected] * Wei Xiao [email protected] 1
Yunnan Engineering Laboratory of Soil Fertility and Pollution Remediation, Yunnan Agricultural University, Kunming 650201, People’s Republic of China
2
Yunnan Institute of Microbiology, Yunnan University, Kunming 650091, People’s Republic of China
3
Key Laboratory of the University in Yunnan Province for International Cooperation in Intercellular Communications and Regulations, Yunnan University, Kunming 650500, People’s Republic of China
4
State Key Laboratory for Conservation and Utilization of Bio‑Resources in Yunnan, Yunnan University, Kunming 650091, People’s Republic of China
Aeromonas hydrophila, a Gram-negative, rod-shaped and facultatively anaerobic bacterium, is ubiquitous in aquatic environments, mud, and a wide range of food [1]. It is notorious as an opportunistic pathogen in amphibians, birds, fish, mammals, and reptiles. A. hydrophila cause serious human infections, such as bacteremia, pneumonia, endocarditis, empyema, arthritis, peritonitis, skin and soft-tissue infections [2–4], an
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