Characterization of 13 polymorphic microsatellite loci in Rimicaris hybisae , a shrimp from deep-sea hydrothermal vents
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TECHNICAL NOTE
Characterization of 13 polymorphic microsatellite loci in Rimicaris hybisae, a shrimp from deep-sea hydrothermal vents Alixandra Jacobson • Sophie Plouviez • Andrew David Thaler • Cindy Lee Van Dover
Received: 8 November 2012 / Accepted: 15 November 2012 Ó Springer Science+Business Media Dordrecht 2012
Abstract Rimicaris hybisae is a deep-sea alvinocaridid shrimp found at hydrothermal vents along the Mid-Cayman Spreading Center. Eleven selectively neutral and unlinked polymorphic microsatellite loci were developed for this species and two additional loci were found to cross-amplify from a related species. Nine loci conformed to Hardy– Weinberg expectations. Seven loci cross-amplified with Chorocaris sp. 2, an alvinocaridid shrimp found at vents in the Southwestern Pacific. Microsatellite loci developed for R. hybisae are being deployed to study connectivity and genetic variability of populations along the Mid-Cayman Spreading Center. Keywords Mid-Cayman Spreading Center Hydrothermal vent Rimicaris hybisae Chorocaris Alvinocarididae
Deep-sea hydrothermal vents are patchily distributed habitats that support large, endemic communities. Studying the fauna of these ecosystems is crucial to understanding how dispersal, isolation, connectivity and evolution function in deep-sea vent environments (e.g., Vrijenhoek 1997). Vents located along the Mid-Cayman Spreading Center (MCSC) are geographically isolated from well-studied vents on the Mid-Atlantic Ridge (Connelly et al. 2012). Two vent fields were confirmed by exploration along the MCSC (Connelly et al. 2012): Von Damm (2,300 m) and Beebe (4,960 m). Population genetic studies using microsatellite markers can
A. Jacobson (&) S. Plouviez A. D. Thaler C. L. Van Dover Division of Marine Science and Conservation, Nicholas School of the Environment, Duke University, 135 Duke Marine Lab Road, Beaufort, NC 28516, USA e-mail: [email protected]
reveal recent connectivity among populations (e.g., Thaler et al. 2011; Teixeira et al. 2012a) and genetic relationships down to the kinship level between individuals of a targeted species (Plouviez et al. 2008). Rimicaris hybisae (Nye et al. 2012), an alvinocaridid shrimp, is known only from the MCSC. R. hybisae occur in dense aggregations at both Von Damm and Beebe. This paper describes 13 microsatellite markers developed to detect R. hybisae population structure at MCSC sites. Rimicaris hybisae genomic DNA was isolated from ethanol-preserved abdomen tissue by Chelex-Proteinase K extraction. Tissue was digested with 120 lg proteinase K (Bioline: Taunton, MA, USA) in 600 ll 10 % Chelex-100 resin (Bio-Rad: Hercules, CA, USA) overnight at 60 °C, heated to 100 °C for 15 min, and centrifuged at 10,000 rpm for 5 min. Genomic DNA was sent to the Institute for Genome Science and Policy at Duke University to be sequenced on a Roche 454 GS-FLX Titanium sequencer. The program msatcommander (v 0.8.2; Faircloth 2008) was used to locate di-, tri-, tetra-, penta- and hexanucleotide repeat motifs in sequences that had signi
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