Isolation and characterization of 26 novel microsatellite loci in the deep-sea shrimp Acanthephyra pelagica
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MICROSATELLITE LETTERS
Isolation and characterization of 26 novel microsatellite loci in the deep-sea shrimp Acanthephyra pelagica Erika Jorquera • Lynne Anstey • Ian Paterson Ellen Kenchington • Daniel E. Ruzzante
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Received: 2 April 2014 / Accepted: 5 April 2014 Ó Springer Science+Business Media Dordrecht 2014
Abstract Acanthephyra pelagica is a widespread deepsea decapod crustacean that is highly abundant and thus, of high ecological significance, within the Sable Gully marine protected area. Despite its importance no genetic analyses have previously been conducted on this species. Here we present a total of 26 novel species-specific microsatellites isolated and characterized in 132 individuals collected from one wild population within the Sable Gully. The number of alleles observed in polymorphic loci ranged from 6 to 18 with an average of 8.1. The observed and expected heterozygosities varied from 0.156 to 0.723 and from 0.156 to 0.913 respectively. These novel microsatellites are a contribution to future studies on A. pelagica and will have a positive impact on the future studies on biology and ecology of this deep-sea species. Keywords Microsatellites Marine protected area Deep-Sea species Acanthephyra pelagica (Decapoda, Caridea) is a crustacean widely distributed in the North and South Atlantic, and South Pacific Oceans. Within the Northwest Atlantic the species is highly abundant in the Sable Gully, a marine Electronic supplementary material The online version of this article (doi:10.1007/s12686-014-0201-0) contains supplementary material, which is available to authorized users. E. Jorquera (&) I. Paterson D. E. Ruzzante Department of Biology, Dalhousie University, Halifax, NS, Canada e-mail: [email protected] L. Anstey E. Kenchington Department of Fisheries and Oceans, Bedford Institute of Oceanography, 1 Challenger Drive, PO Box 1006, Dartmouth, NS B2Y 4A2, Canada
protected area (MPA) off the Atlantic coast of Canada (44°N 59°W). Although the species is not exploited and thus can be thought of no economic value, its high biomass in areas of high biological productivity suggest it is of high ecological importance (MacIsaac et al. 2013). The Sable Gully is the largest submarine canyon off the coast of eastern North America and is recognized for being an area of high biodiversity and biological productivity (Fisheries and Oceans Canada, 1998). A. pelagica is a deep-sea species with a bathymetric distribution ranging between 200 and 2000 m depth, exhibiting daily vertical migrations, this behavior is thought to play a major role in the transfer of energy between pelagic and benthic environments. (MacIsaac et al. 2013). A total of 26 novel species specific microsatellites were developed to examine population structure and analyze patterns of paternity (Table 1). No other microsatellites have previously been developed for this species. The microsatellites presented here were developed with individuals sampled within the Sable Gully MPA on the Atlantic coast of Canada. Acanthephyra pelagica indivi
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