Chryseobacterium formosus sp. nov., a bacterium isolated from an ancient tree trunk
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ORIGINAL PAPER
Chryseobacterium formosus sp. nov., a bacterium isolated from an ancient tree trunk Shahina Akter1 · Hien T. T. NGO1 · Juan Du1 · KyungHwa Won1 · Hina Singh1 · Chang Shik Yin2 · MooChang Kook3 · Tae‑Hoo Yi1
Received: 19 May 2015 / Revised: 30 June 2015 / Accepted: 14 July 2015 © Springer-Verlag Berlin Heidelberg 2015
Abstract A Gram-reaction-negative, non-motile and rodshaped bacterium, designated as THG-DN3.6T, was isolated from an ancient tree trunk from Republic of Korea. On the basis of 16S rRNA gene sequence analysis, strain THGDN3.6T was shown to belong to the genus Chryseobacterium and the highest similarity to Chryseobacterium indoltheticum LMG 4025T (97.2 %) and the closest phylogenetic relatives were Chryseobacterium scophthalmum (96.8 %), Chryseobacterium piscium (96.7 %) and Chryseobacterium balustinum KCTC 2903T (96.3 %). The DNA G + C content of the isolate was 33.2 mol %. The predominant isoprenoid quinone was menaquinone-6. The major fatty acids were iso-C15:0, summed feature 3 (C16:1ω7c and/or C16:1ω7t and/or iso-C15:0 2-OH), iso-C17:1ω9c and iso-C17:0 3-OH. Communicated by Erko Stackebrandt. Shahina Akter and Hien T. T. NGO have contributed equally to this work. The NCBI GenBank accession number for the 16S rRNA gene sequence of strain THG-DN3.6T is KM035938. Electronic supplementary material The online version of this article (doi:10.1007/s00203-015-1137-9) contains supplementary material, which is available to authorized users. * Tae‑Hoo Yi [email protected] 1
Department of Oriental Medicinal Material and Processing College of Life Science, Kyung Hee University, 1 Seocheon, Kihung, Yongin 446‑701, Gyeonggi‑do, Republic of Korea
2
Department of Acupuncture Merdian Science Research Center, College of Korean Medicine, Kyung Hee University, Yongin, Republic of Korea
3
Department of Marine Biotechnology, Anyang University, Incheon 417‑833, Republic of Korea
The major polar lipids of strain THG-DN3.6T were phosphatidylethanolamine. The mean DNA–DNA relatedness of strain THG-DN3.6T to C. indoltheticum LMG 4025T was 52 ± 0.5 %. Based on the results of polyphasic characterization, strain THG-DN3.6T represented a novel species within the genus Chryseobacterium, for which the name Chryseobacterium formosus sp. nov. is proposed. The type strain is THG-DN3.6T (=KCTC 42606 = CCTCC AB 2015118). The NCBI GenBank accession number for the 16S rRNA gene sequence of strain THG-DN3.6T is KM035938. Keywords Chryseobacterium formosus · Gram-reactionnegative · 16S rRNA · Menaquinone-6
Introduction The genus Chryseobacterium, belonging to the family Flavobacteriaceae, class Flavobacteria, phylum Bacteroidetes, was first described by Vandamme et al. (1994) and subsequently emended by Kämpfer et al. (2009) and Wu et al. (2013). As previous reports describe, members representing the genus Chryseobacterium are Gram-reaction-negative, rod-shaped, flexirubin-type pigments, menaquinone-6 (MK6) as the major predominant respiratory quinone and the range of DNA G + C content with 29–41.1 mol
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