Proposal of Thermoactinomyces mirandus sp. nov., a filamentous, anaerobic bacterium isolated from a biogas plant
- PDF / 1,136,284 Bytes
- 10 Pages / 547.087 x 737.008 pts Page_size
- 54 Downloads / 198 Views
(0123456789().,-volV) ( 01234567 89().,-volV)
ORIGINAL PAPER
Proposal of Thermoactinomyces mirandus sp. nov., a filamentous, anaerobic bacterium isolated from a biogas plant Mira Mutschlechner . Nina Lackner . Rudolf Markt . Willi Salvenmoser . Christopher A. Dunlap . Andreas O. Wagner
Received: 2 October 2020 / Accepted: 4 November 2020 Ó The Author(s) 2020
Abstract We isolated a filamentous, thermophilic, and first anaerobic representative of the genus Thermoactinomyces, designated strain AMNI-1T, from a biogas plant in Tyrol, Austria and report the results of a phenotypic, genetic, and phylogenetic investigation. Strain AMNI-1T was observed to form a white branching mycelium that aggregates into pellets when grown in liquid medium. Cells could primarily utilize lactose, glucose, and mannose as carbon and energy sources, with acetate accelerating and yeast extract being mandatory for growth. The optimum growth temperature and pH turned out to be 55 °C and pH 7.0, respectively, with an optimum NaCl concentration of 0–2% (w/v). 16S rRNA gene sequence comparison
indicated that the genetic relatedness between strain AMNI-1T and Thermoactinomyces intermedius, Thermoactinomyces khenchelensis, and Thermoactinomyces vulgaris was less than 97%. The G ? C content of the genomic DNA was 44.7 mol%. The data obtained suggest that the isolate represents a novel and first anaerobic species of the genus Thermoactinomyces, for which the name Thermoactinomyces mirandus is proposed. The type strain is AMNI1T (= DSM 110094T = LMG 31503T). The description of the genus Thermoactinomyces is emended accordingly. Keywords Hydrogen production Thermophilic NiFe Hydrogenase Hyp Novobiocin Thermoactinomycetaceae Fermentation
Mira Mutschlechner and Nina Lackner have contributed equally to this work. M. Mutschlechner (&) N. Lackner R. Markt A. O. Wagner Department of Microbiology, Universita¨t Innsbruck, Technikerstraße 25d, 6020 Innsbruck, Austria e-mail: [email protected] W. Salvenmoser Department of Zoology, Universita¨t Innsbruck, Technikerstraße 25, 6020 Innsbruck, Austria C. A. Dunlap Crop Bioprotection Research Unit, Agricultural Research Service, US Department of Agriculture, National Center for Agricultural Utilization Research, Peoria, IL 61604, USA
Introduction The genus Thermoactinomyces within the family Thermoactinomycetaceae was initially described by Tsilinsky (1899), with the first known representative Thermoactinomyces vulgaris being isolated from decaying straw and manure. Since then, several species of Thermoactinomyces have been isolated from a variety of habitats (Bezuidt et al. 2016). In 2005, several former Thermoactinomyces species were reclassified in new genera Laceyella, Thermoflavimicrobium and Seinonella (Yoon et al. 2005).
123
Antonie van Leeuwenhoek
Currently, the genus comprises four validly described species including Thermoactinomyces vulgaris (Tsilinsky 1899), Thermoactinomyces intermedius (Kurup et al. 1980), Thermoactinomyces daqus (Yao et al. 2014), and Thermoact
Data Loading...
