Acetatifactor muris gen. nov., sp. nov., a novel bacterium isolated from the intestine of an obese mouse
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ORIGINAL PAPER
Acetatifactor muris gen. nov., sp. nov., a novel bacterium isolated from the intestine of an obese mouse Nora Pfeiffer • Charles Desmarchelier • Michael Blaut • Hannelore Daniel • Dirk Haller Thomas Clavel
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Received: 10 March 2012 / Revised: 10 May 2012 / Accepted: 11 May 2012 / Published online: 4 June 2012 Ó Springer-Verlag 2012
Abstract We used selective agar media for culturing bacteria from the caecum of mice fed a high calorie diet. In addition to the isolation of Enterobacteriaceae growing on a medium containing cholesterol and bile salts, we focused on the characterization of strain CT-m2T, which, based on 16S rDNA analysis, did not appear to correspond to any currently described organisms. The isolate belongs to the Clostridium cluster XIV and is most closely related to members of the Lachnospiraceae, including the genera Anaerostipes, Blautia, Butyrivibrio, Clostridium, Coprococcus, Eubacterium, Robinsoniella, Roseburia, Ruminococcus and Syntrophococcus (B90 % similarity). Strain CT-m2T is a non-motile Gram-positive rod that does not form spores and has a G ? C content of DNA of 48.5 %. Cells grow under strictly anoxic conditions (100 % N2) and Communicated by Erko Stackebrandt. The GenBank accession number of the 16S rRNA gene sequence of strain CT-m2T is HM989805.
Electronic supplementary material The online version of this article (doi:10.1007/s00203-012-0822-1) contains supplementary material, which is available to authorized users. N. Pfeiffer M. Blaut Gastrointestinal Microbiology, German Institute of Human Nutrition Potsdam-Rehbru¨cke, Nuthetal, Germany C. Desmarchelier H. Daniel Molecular Nutrition Unit, ZIEL, Research Centre for Nutrition and Food Sciences, Technische Universita¨t Mu¨nchen, Freising-Weihenstephan, Germany Present Address: C. Desmarchelier Faculte´ de Me´decine, INSERM, UMR 1062 « Nutrition, Obesity and Risk of Thrombosis »/INRA, UMR1260/Universite´ d’Aix-Marseille, Marseille, France
produce acetate and butyrate after growth in reduced WCA broth. In contrast to related species, the new bacterium does not metabolize glucose and is positive for phenylalanine arylamidase, and its major cellular fatty acid is C14:0. Based on phylogenetic and phenotypic studies, the isolate merits recognition as a member of a novel genus and species, for which the name Acetatifactor muris is proposed. The type strain is CT-m2T (= DSM 23669T = ATCC BAA-2170T). Keywords Mouse intestinal microbiota Diet-induced obesity Firmicutes Clostridia Acetatifactor muris
Introduction The mammalian gut microbiota is dominated by over 1,000 species of strictly anaerobic bacteria, predominantly Grampositive type cells belonging to the phylum Firmicutes (Ley et al. 2008). The study of such highly diverse ecosystems is partly hampered by the fact that most gut bacteria ([60 %) have no representative strains yet in culture (Goodman et al. 2011), highlighting the importance of isolating novel bacterial species for further in vitro D. Haller T. Clavel Biofunctionality, ZIEL, Research Centr
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