Colorimetric determination of tyrosinase based on in situ silver metallization catalyzed by gold nanoparticles
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ORIGINAL PAPER
Colorimetric determination of tyrosinase based on in situ silver metallization catalyzed by gold nanoparticles Hui Liu 1 & Bowen Liu 1 & Pengcheng Huang 1,2 & Yangyang Wu 1 & Fang-Ying Wu 1,2
&
Lihua Ma 3
Received: 24 June 2020 / Accepted: 24 July 2020 # Springer-Verlag GmbH Austria, part of Springer Nature 2020
Abstract Gold nanoparticles (AuNPs) catalyze the mild reaction between the weak reducing agent kojic acid (KA) and silver ions (Ag+) to form Au@Ag bimetallic NPs by the combination of the intrinsic catalysis with plasmonic properties This is proposed as a novel optical assay to determine the tyrosinase (TYRase) concentration. The nanoparticles have been characterized by UV-vis spectroscopy, transmission electron microscope (TEM) images, and X-ray photoelectron spectroscopy (XPS). The sensing mechanism is based on the fact that KA binds to TYRase by chelating with dicopper active site of TYRase and the introduction of TYRase restrains the Au@Ag bimetallic NP formation by the precedent binding with KA. A clear color variation from yellow to pink and UV-vis spectral changes are observed at the optimal wavelength of 410 nm. The assay works in the range 0.13~0.73 U mL−1 with a detection limit (LOD) of 0.019 U mL−1. The impact from matrix interfering substances including glucose, uric acid, common oxidases, and amino acids is negligible. The applicability is demonstrated by quantitative determination of TYRase in human serum samples with 74 to 89% recovery and RSD less than 4.0%, which accords with the level for bio-sample analysis. Keywords Silver deposition . Visual determination . Tyrosinase . Core-shell nanoparticles
Introduction Tyrosinase (TYRase), as a copper-containing polyphenol oxidase enzyme, directly participates in the synthesis of pigments in organisms [1]. The abnormal level of TYRase in human results in skin and nervous system disease such as vitiligo, melanoma cancer, and Parkinson’s syndrome [2–6]. It is crucial to develop the methods to recognize TYRase level Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00604-020-04463-9) contains supplementary material, which is available to authorized users. * Pengcheng Huang [email protected] * Fang-Ying Wu [email protected] 1
College of Chemistry, Nanchang University, Nanchang 330031 China
2
Jiangxi Province Key Laboratory of Modern Analytical Science, Nanchang University, Nanchang 330031 China
3
College of Science and Engineering, University of Houston at Clear Lake, 2700 Bay Area Blvd Houston TX 77058 USA
with high sensitivity in biological samples. To date, a number of technologies have been established for TYRase sensing, including colorimetry [7–9], fluorescence spectroscopy [10–12], surface-enhanced Raman scattering [13], electrochemistry [14, 15], and bioluminescence [16]. The colorimetry has been valued as one of the potential methods because of its convenience, speediness, and inexpensiveness. Noble metal nanoparticles, such as gold and silver nanoparticles (Au and Ag NPs),
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