Combination of Adsorption and Cellulose Derivative Membrane Coating for Efficient Immobilization of Laccase
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Combination of Adsorption and Cellulose Derivative Membrane Coating for Efficient Immobilization of Laccase Rong Wu 1,2 & Feixiang Liu 1,2 & Qihui Dong 1,2 & Yanyan Huang 2 & Yibin Qiu 2 & Yiyi Sun 2 & Erzheng Su 1,2,3 Received: 23 June 2020 / Accepted: 29 September 2020/ # Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract
Immobilization of enzyme based on combination of adsorption and cellulose derivative membrane coating was established in this work for the first time. Laccase, a commonly used enzyme in varied fields, was chosen as the model enzyme to demonstrate this method. After investigating operational conditions, the optimal process was obtained as follows: diatomite or HPD-417 as the adsorption carrier, 0.5% (w/v) methylcellulose (40,000~50,000) acetone solution as the coating solution, 0.75% (w/v) polyethylene glycol or maltose as the protective agent, and drying at 4 °C for 9 h. Under the optimal conditions, the residual activities of diatomite and HPD-417 immobilized laccase reached 99.33% and 94.15%, respectively. The study on properties showed that the immobilized laccases held high pH tolerance and thermal stability. The immobilized laccases were further applied to the indigo decolorization and 2, 4-dichlorophenol degradation. They showed high catalytic efficiency and could be reused for several batches. On the whole, the immobilization method developed in this work can effectively avoid the inactivation of laccase during immobilization and improve the stability of immobilized laccase. The laccase immobilized by this method shows obvious potential for environmental governance. Keywords Immobilization . Adsorption . Cellulose derivative coating . Laccase . Decolorization . Degradation Electronic supplementary material The online version of this article (https://doi.org/10.1007/s12010-02003446-z) contains supplementary material, which is available to authorized users.
* Erzheng Su [email protected]
1
Co-innovation Center for the Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, China
2
Department of Food Science and Technology, College of Light Industry and Food Engineering, Nanjing Forestry University, Nanjing 210037, China
3
Co-Innovation Center for Efficient Processing and Utilization of Forest Products, Nanjing Forestry University, Nanjing 210037, China
Applied Biochemistry and Biotechnology
Introduction Owning to the advantages such as high specificity, high catalytic efficiency, and mild reaction conditions, enzyme catalytic reactions play important roles in different fields such as food, chemical, pharmaceutical, and environmental [1–3]. The keys to enzyme-catalyzed reactions lie in enzyme stability and enzyme activity [4, 5]. However, the poor stability of free enzymes leads to the difficulties in recovery and reusability, which greatly limits industrial applications of enzymes [6, 7]. Therefore, a promising approach to solve the above problems is using enzyme immobilization technology, and its appearance overcomes many sh
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