Immobilization of Laccase on Magnetic Nanoparticles and Application in the Detoxification of Rice Straw Hydrolysate for
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Immobilization of Laccase on Magnetic Nanoparticles and Application in the Detoxification of Rice Straw Hydrolysate for the Lipid Production of Rhodotorula glutinis Liang Yin 1 & Jiamin Chen 1 & Weixiao Wu 1 & Zhikang Du 1 & Yanqing Guan 1 Received: 27 July 2020 / Accepted: 9 November 2020/ # Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract
The production of microbial lipid using lignocellulosic agroforestry residues has attracted much attention. But, various inhibitors such as phenols and furans, which are produced during lignocellulosic hydrolysate preparation, are harmful to microbial lipid accumulation. Herein, we developed a novel detoxification strategy of rice straw hydrolysate using immobilized laccase on magnetic Fe3O4 nanoparticles for improving lipid production of Rhodotorula glutinis. Compared with free laccase, the immobilized laccase on magnetic nanoparticles showed better stability, which still retained 76% of original activity at 70 °C and 56% at pH 2 for 6 h. This immobilized laccase was reused to remove inhibitors in acid-pretreated rice straw hydrolysate through recycling with external magnetic field. The results showed that most of phenols, parts of furans, and formic acids could be removed by immobilized laccase after the first batch. Notably, the immobilized laccase exhibited good reusability in repeated batch detoxification. 78.2% phenols, 43.8% furfural, 30.4% HMF, and 16.5% formic acid in the hydrolysate were removed after the fourth batch. Furthermore, these detoxified rice straw hydrolysates, as substrates, were applied to the lipid production of Rhodotorula glutinis. The lipid yield in detoxified hydrolysate was significantly higher than that in undetoxified hydrolysate. These findings suggest that the immobilized laccase on magnetic nanoparticles has a potential to detoxify lignocellusic hydrolysate for improving microbial lipid production. Keywords Immobilized laccase . Magnetic nanoparticles . Detoxification . Rice straw hydrolysate . Lipid production
* Liang Yin [email protected]
1
School of Life Science, South China Normal University, Guangzhou 510631, China
Applied Biochemistry and Biotechnology
Introduction Biofuel, such as bioethanol, biodiesel, is becoming increasingly important due to its sustainability and renewability [1]. Microbial lipid can be processed to biodiesel by transesterification; therefore, it has received increasing attention. Some microorganisms, such as microalgae, oleaginous yeasts, and filamentous fungi, are able to accumulate high content of intracellular lipid (single-cell oil, SCO) in a short period [2, 3]. However, high production cost of microbial lipid limits the commercial application. The oleaginous microorganisms require various fermentation substrates in lipid production. The cost of microbial lipid is mainly attributed to fermentation materials. Seeking low-cost and abundant substrates is a crucial strategy to reduce the production cost of microbial lipid. Compared with other raw materials, lignocellulosic biomas
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