Comparison of the regenerative effects of bone marrow/adipose-derived stem cells in the Asherman model following local o
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REPRODUCTIVE PHYSIOLOGY AND DISEASE
Comparison of the regenerative effects of bone marrow/adipose-derived stem cells in the Asherman model following local or systemic administration Farhad Monsef 1 & Tayebe Artimani 1,2 & Zohreh Alizadeh 1,2 & Mahdi Ramazani 1,2 & Ghasem Solgi 3 & Mahnaz Yavangi 2 & Sara Soleimani Asl 1,2 Received: 4 January 2020 / Accepted: 9 June 2020 # Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract Purpose Cell therapy is a promising strategy for the treatment of Asherman’s syndrome (AS), but the origin of these cells and injection route influence the therapeutic effect and complications of cell therapy. Herein, we compared the effects of systemic or local intrauterine injection of bone marrow or adipose-derived mesenchymal stem cells (BMSCs/AMSCs) on the endometrium in a rat model of AS. Methods After induction of AS in adult Wistar rats, the CM-Dil-positive BMSCs or AMSCs were injected either locally or intravenously. After 3 weeks, endometrial thickness, collagen deposition, cell migration, and VEGF expression were evaluated using histochemistry/immunofluorescence studies. Results In all stem cell-treated groups, an ameliorative effect on the damaged endometrium was noted. Collagen deposition diminished in both groups (IV and local injection) compared to the AS model. In rats injected locally with MSC, fibrosis decreased compared to the other groups. Moreover, endometrial thickness increased in the groups that received local injection of BMSCs and AMSCs more than the IV-transplanted AMSCs group. Immunofluorescent staining demonstrated that although the systemic transplantation of BMSCs was more effective than the other groups on VEGF expression, it led to the lowest number of CM-Dil+ stem cells in the damaged endometrium. Conclusion Stem cell transplantation may reconstruct the damaged endometrium, but it is recommended to select the most effective stem cells and injection route. Because the removal of the fibrosis and the replacement of the epithelia cells is an effective therapeutic strategy for AS, in this study, we conclude that the local injection of AMSCs is more appropriate than BMSCs to treat AS. Keywords Asherman’s syndrome . Bone marrow-derived mesenchymal stem cell . Adipose-derived mesenchymal stem cell . Endometrium
Introduction Asherman’s syndrome (AS) is a typical gynecological disorder that is characterized by intrauterine adhesion, fibrosis, and * Sara Soleimani Asl [email protected] 1
Department of Anatomy, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran
2
Endometrium and Endometriosis Research Centre, Hamadan University of Medical Sciences, Hamadan, Iran
3
Department of Immunology, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran
loss of luminal epithelial cells [1]. The incidence of AS is up to 30% in women who have undergone dilation and curettage after a late spontaneous abortion, and 13% in women who have had a pregnancy termination in the first trimester [2]. The removal o
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