Caldimonas hydrothermale sp. nov., a novel thermophilic bacterium isolated from roman hot bath in south Tunisia
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ORIGINAL PAPER
Caldimonas hydrothermale sp. nov., a novel thermophilic bacterium isolated from roman hot bath in south Tunisia Hanene Bouraoui · Imen Boukari · Jean Pierre Touzel · Michael O’Donohue · Mohamed Manai
Received: 3 December 2009 / Revised: 30 March 2010 / Accepted: 15 April 2010 / Published online: 5 May 2010 © Springer-Verlag 2010
Abstract A polyphasic approach was used to characterize a bacterium, HAN-85T, isolated from thermal water in natural thermal spring at Tozeur, an oasis in southwest Tunisia. The novel isolate was thermophilic, strictly aerobic and amylolytic bacterium, which stained Gram negative. Cells were short rods motile by means of a single polar Xagellum. Their optimum temperature and pH required for growth were 55°C and pH 7, respectively. Comparative 16S rRNA gene sequence analyses showed that strain HAN-85T belonged to the genus Caldimonas, with highest sequence similarity to the type strains Caldimonas manganoxidans and Caldimonas taiwanensis. DNA–DNA hybridization
Communicated by Erko Stackebrandt.
measurements revealed low DNA relatedness (35.2– 44.5%) between the novel isolate and its closest relative, C. manganoxidans. The major cellular fatty acid components were 16:0, 17:0 cyclo and summed feature 3. The DNA G+C content was 68.3 mol%. Taken together, the results of DNA–DNA hybridization, fatty acids proWle, physiological tests and biochemical analyses have allowed the genotypic and phenotypic diVerentiation of the isolate from currently recognized Caldimonas species. Therefore, we suggest that this isolate is a novel species within the genus Caldimonas and propose that it should be named Caldimonas hydrothermale sp. nov. The type strain is HAN-85T (=DSM 18497T =LMG 23755T). The Gen Bank/Embl/DDBJ accession number for the 16S rRNA gene sequence of strain DSM 18497T is AM283038.
Electronic supplementary material The online version of this article (doi:10.1007/s00203-010-0576-6) contains supplementary material, which is available to authorized users.
Keywords Caldimonas · Thermophile · Amylolytic bacterium · Hammam · Tunisian thermal spring
H. Bouraoui (&) · M. Manai Laboratoire de Biochimie et Biologie Moléculaire, Faculté des Sciences de Tunis, Campus Universitaire El-Manar, 2092 Tunis, Tunisie e-mail: [email protected] I. Boukari · J. P. Touzel INRA, UMR FARE INRA-URCA 614, 8, rue Gabriel-Voisin, BP 316, 51688 Reims Cedex 02, France M. O’Donohue Université de Toulouse, INSA, UPS, INP, LISBP, 135 Avenue de Rangueil, 31077 Toulouse, France M. O’Donohue INRA, UMR792 Ingénierie des Systèmes Biologiques et des Procédés, 31400 Toulouse, France M. O’Donohue CNRS, UMR5504, 31400 Toulouse, France
Introduction Thermophiles dominate the deepest and shortest branches of the Bacteria and Archaea domains in the tree of life, suggesting that they are likely ancestors of Earth’s contemporary microbial populations (Caetano 2002; Schwartzman and Lineweaver 2004). Study of such organisms will enhance our understanding of survival and adaptation strategies in extreme environments an
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