Current Topics in Microbiology and Immunology
- PDF / 30,487,169 Bytes
- 151 Pages / 481.89 x 691.654 pts Page_size
- 71 Downloads / 222 Views
w. ATber, Basle
. S. Falkow, Seattle· W. Henle, Philadelphia
P.H. Hofschneider, Martinsried . lH. Humphrey, London J. Klein, TUbingen . P. Koldovsky, DUsseldorf· H. Koprowski, Philadelphia· O. Maal0e, Copenhagen· F. Melchers, Basle R. Rott, GieBen . H.G. Schweiger, Ladenburg/Heidelberg L. Syrucek, Prague· P.K. Vogt, Los Angeles
With 32 Figures
Springer-Verlag Berlin Heidelberg New York 1980
ISBN-13:978-3-642-67719-9 e- ISBN-13:978-3-642-67717-5 DOl 10.1007/978-3-642-67717-5
This work is subject to copyright Ail rights are reserved, whether the whole or part of the material is concerned, specifically those of translation, reprinting, re-use of illustration broadcasting, reproduction by photocopying machine or similar means, and storage in data banks. Under § 54 of the German Copyright Law where copies are made for other than private use, a fee is payable to the publisher, the amount of the fee to be determined by argeement with the publisher. © by Springer-Verlag Bertin Heidelberg 1980. Softcover reprint of the hardcover 1st edition 1980 Library of Congress Catalog Card Number 15-12910. The use of registered names, trademarks, etc. in this publication, does nor imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. Typesetting: Fotosatz Service Weihrauch, Wiirzburg 2121/3321-543210
Table of Contents
J.H. Miller: Genetic Analysis of the lac Repressor.
1
H.-D. Klenk und R. Rott: Cotranslational and Posttranslational Processing of Viral Glycoproteins . . 19 June Rothman Scott: Immunity and Repression in Bac. . . . . . . . 49 teriophages PI and P7 Bodil Norrild: Immunochemistry of Herpes Simplex Virus ....... . 67 Glycoproteins H. Becht: Infectious Bursal Disease Virus
107
D. Mergenhagen: Circadian Rhythms in Unicellular Organisms . . . . . . . . 123
Indexed in Current Contents
Genetic Analysis of the lac Repressor JEFFREY
H.
MILLER *
Introduction. . . 2 The Genetic System 2.1 Deletion Mapping. 2.2 Deletions in the I Region 2.3 Correlation of the Genetic and Physical Map. 3 Mutations Affecting the Amino-Terminal Region of the Repressor: 3.1 Mutations Affecting Aggregation. 3.2 f Mutations. . . . 3.3 Tight-Binding Mutants . . . . 3.4 Temperature Effects . . . . . 3.5 Altered Repressors Generated by Suppressed Nonsense Mutations 4 Combined Analysis 5 Conclusions. References
r d Mutations
2 2 3 3 5 7 8 9
11
12 12 15 15
1 Introduction Understanding how proteins function is an essential part of molecular biology, since specific proteins or enzymes ultimately mediate all cellular processes. Umaveling the mysteries of prokaryotic and eukaryotic cells will require identification of the relevant proteins, and understanding how they work. Genetics is a powerful tool for the investigation of protein structure, as exemplified by studies of the lac! gene of Eschericha coli, and its protein product, the lac repressor. This review covers recent advances in our understanding of lac repressor. More detai
