De novo assembly and comparative analysis of the complete mitochondrial genome sequence of the pistachio psyllid, Agonos
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ORIGINAL RESEARCH ARTICLE
De novo assembly and comparative analysis of the complete mitochondrial genome sequence of the pistachio psyllid, Agonoscena pistaciae (Hemiptera: Aphalaridae) Lida Fekrat 1
&
Mohammad Zakiaghl 1 & Manpreet K. Dhami 2 & Yue Zhang 3 & Marzieh Koohkanzade 1
Received: 19 July 2020 / Accepted: 9 October 2020 # African Association of Insect Scientists 2020
Abstract The pistachio psyllid, Agonoscena pistaciae, is one of the most deleterious pests of pistachio trees. Deciphering genetic diversity and population structure is a prerequisite for developing efficacious suppression protocols and successful management strategies for any insect pest including A. pistaciae. Mitochondrial genome sequencing furnishes valuable information about insect evolution and diversity within and among populations. Here, we assembled the first complete mitochondrial genome of the pistachio psyllid high-throughput next generation sequencing. The circular mitochondrial genome of A. pistaciae was 15,346 bp in length encoding 37 genes, including 13 protein-coding genes (PCGs), 2 rRNA genes and 22 tRNA genes, with a long noncoding region (putative control regions). The A. pistaciae mitogenome was in same typical set and arrangement as that of the ancestral insect. The mitochondrial genome of A. pistaciae was compared to all 6 publically available mitogenomes of other Psylloidea species. The conservation of the gene arrangements and identical gene ordering among all psyllid species could suggest a structurally common ancestral mitogenome shared across Psylloidea. Phylogenetic analysis based on the 13 PCGs conformed the existing morphological based taxonomy and showed the clustering of the two Aphalaridae. Whole mitochondrial genome of A. pistaciae could be utilized in subsequent population genetic studies of the species and also add to a deeper understanding of the phylogenetic research links within the subfamily Psylloidea. Keywords Agonoscena pistaciae . Mitochondrial genome . Phylogenetic analysis . Aphalaridae . Psylloidea
Introduction Pysllids or jumping plant lice are a group of inconspicuous, phloem-feeding insects, with eight families and almost 4000 species worldwide (Cho et al. 2017; Ouvrard 2020). As vectors of significant plant pathogens (Perilla-Henao and Casteel 2016; Electronic supplementary material The online version of this article (https://doi.org/10.1007/s42690-020-00332-3) contains supplementary material, which is available to authorized users. * Lida Fekrat [email protected] 1
Department of Plant protection, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran
2
Biodiversity & Conservation, Manaaki Whenua Landcare Research, Lincoln, New Zealand
3
Department of Entomology, College of Plant Protection, China Agricultural University, Beijing 100193, China
Wang et al. 2017), salient pests in agriculture and forestry and also prospective control agents of exotic invasive plants, psyllids have recently received great attention globally (Burckhardt and Ouvrard 2012; Martoni and Arms
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