The complete mitochondrial genome sequence of Changbai Mountains wild boar (Cetartiodactyla: Suidae)
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TECHNICAL NOTE
The complete mitochondrial genome sequence of Changbai Mountains wild boar (Cetartiodactyla: Suidae) Zhen‑Dong Tan1 · Lin‑Yuan Shen1 · Xiao Cheng1 · Mai‑Lin Gan1 · Shun‑Hua Zhang1 · Li Zhu1
Received: 6 May 2017 / Accepted: 8 May 2017 © Springer Science+Business Media Dordrecht 2017
Abstract The Changbai Mountains wild boar (Cetartiodactyla: Suidae) is an omnivorous, gregarious and nonhibernating animal that inhabits in Jilin province of China and is threatened by poaching and deforestation. In this study, we provide the first report of the complete mitogenome of Changbai Mountains wild boar. The total length of mitochondrial genome is 16,720 bp and consists of 22 transfer RNA genes, 13 protein-coding genes, two ribosomal RNA genes, and a major non-coding control region (D-loop region). The D-loop region contains one repeat motif. The full complement of genes in the Changbai Mountains wild boar are located on the H-strand, except for the ND6 gene and eight tRNA genes that are encoded on the L-strand. Phylogenetic analysis based on combining 13 protein-coding gene sequences of several species showed that it is closely related to genus Sus scrofa in north China. The mitochondrial genome of Changbai Mountains wild boar is useful for population genetics and phylogenetic analysis. Keywords Changbai Mountains wild boar · Genome · Mitochondria The Changbai Mountains wild boar is a native wild boar that inhabits in the forests of Jilin Changbai Mountains in China. Compared to the other wild boar subspecies Zhen-Dong Tan and Lin-Yuan Shen have contributed equally to this work. * Li Zhu [email protected] 1
College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, Sichuan, China
in China (South subspecies and Northwest subspecies) (Zhang et al. 1986), the Changbai Mountains wild boar has a higher proportion of lean meat, stronger adaptability and excellent meat quality, but lower reproductive capacity. Since the government banned hunting and implemented the conversion of cropland to forest, the Changbai Mountains wild boar population has increased significantly and largescale production has improved the local economy (Cui and Wang 2001). The mitochondrial genome of Changbai Mountains pig provides important data on germplasm. To preserve this genetic resource and thus contribute to its conservation, we reported the first mitochondrial genome sequence and have deposited this sequence in GenBank (accession number KP765605). The Changbai Mountains wild boar sample was collected from Baishan Country, Jilin Province, China (41.47°N, 127.18°E). The specimen (DNA and tissue) was deposited in the Museum of Sichuan Agricultural University (Voucher numbers MSAU-20151404). Total genomic DNA was extracted from ethanol-preserved ear tissue using the DNeasy Blood & Tissue Kit (Qiagen, Crawley, UK) according to the manufacturer’s protocol. Primers to amplify the Tibetan pig (GenBank accession number KC493607) mitochondrial genome were used to amplify the mitochondrial genome of Chan
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