Degradation potential and diversity of oil-degrading bacteria isolated from the sediments of the Jiaozhou Bay, China
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Degradation potential and diversity of oil-degrading bacteria isolated from the sediments of the Jiaozhou Bay, China Wei Gao1, 2†, Xiangxing Gao2, 3†, Tiezhu Mi4, 5, 6, Bin Han2, Yiran Zhang2, Xinzi Li2, Xiaofei Yin2, Chengjun Sun2, Qian Li2, Zhisong Cui2, Xiao Luan2, Zhigang Yu5, 6, Li Zheng2, 6* 1 College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China 2 Marine Ecology Research Center, First Institute of Oceanography, Ministry of Natural Resources, Qingdao 266061,
China 3 National Deep Sea Center, Qingdao 266237, China 4 College of Environmental Science and Engineering, Ocean University of China, Qingdao 266100, China 5 Key Laboratory of Marine Chemical Theory and Technology, Ministry of Education, Qingdao 266100, China 6 Laboratory for Marine Ecology and Environmental Science, Pilot National Laboratory for Marine Science and
Technology (Qingdao), Qingdao 266237, China Received 2 February 2018; accepted 22 March 2018 © Chinese Society for Oceanography and Springer-Verlag GmbH Germany, part of Springer Nature 2019
Abstract
A great deal of oil contaminated the shoreline by the Qingdao oil pipeline explosion in 2013. The four oildegrading consortia were enriched from sediment samples with crude oil as sole carbon and energy sources. The biodiversity and community analysis showed that the Luteibacter, Parvibaculum and a genus belonging to Alcanivoracaceae were found predominant bacteria in the four consortia, which belonged to Proteobacteria. Nine strains exhibiting distinct 16S rRNA gene sequences were isolated from the consortia. These strains were identified to eight genera based on 16S rRNA gene sequences. Five of the nine strains degraded more than 30% of the crude oil in two weeks by gravimetric method. From the analysis of GC-MS, most of the isolated strains tended to degrade n-alkanes rather than PAHs. Five strains showed high degrading ability of the total n-alkanes. Only Strain D2 showed great PAHs degrading ability and the degrading rates ranged from 34.9% to 77.5%. The sequencing analysis of the oil-degrading consortia confirmed that the genus of Alcanivorax was one of the dominant bacteria in Consortia A and E and Strain E4 might be one of the dominant bacteria. The strains obtained in this study demonstrated the potential for oil bioremediation in oil-contaminated beach ecosystems. Key words: oil spill, biodegradation, microbial consortium, dominant bacteria Citation: Gao Wei, Gao Xiangxing, Mi Tiezhu, Han Bin, Zhang Yiran, Li Xinzi, Yin Xiaofei, Sun Chengjun, Li Qian, Cui Zhisong, Luan Xiao, Yu Zhigang, Zheng Li. 2019. Degradation potential and diversity of oil-degrading bacteria isolated from the sediments of the Jiaozhou Bay, China. Acta Oceanologica Sinica, 38(6): 54–64, doi: 10.1007/s13131-019-1353-2
1 Introduction Millions of liters of petroleum hydrocarbons from both natural and anthropogenic sources contaminated the marine environment annually (Hassanshahian et al., 2012). Bioremediation technologies to degrade these pollutants had long been investigated in laborato
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