Sphingorhabdus pacificus sp. nov., isolated from sandy sediments of the Sea of Japan seashore
- PDF / 277,378 Bytes
- 7 Pages / 595.276 x 790.866 pts Page_size
- 4 Downloads / 165 Views
ORIGINAL PAPER
Sphingorhabdus pacificus sp. nov., isolated from sandy sediments of the Sea of Japan seashore Lyudmila A. Romanenko · Naoto Tanaka · Vassilii I. Svetashev · Valery V. Mikhailov
Received: 1 June 2014 / Revised: 5 August 2014 / Accepted: 21 August 2014 © Springer-Verlag Berlin Heidelberg 2014
Abstract An aerobic, Gram-negative, yellow-pigmented, non-motile rod-shaped bacterium designated KMM 9574T was isolated from a sand sediment sample collected from the Sea of Japan seashore. Comparative 16S rRNA gene sequence analysis showed that strain KMM 9574T belonged to the genus Sphingorhabdus sharing a highest sequence similarity to Sphingorhabdus marina JCM 14161T 96.8 %. Strain KMM 9574T was characterized by the major ubiquinone Q-10, and by the predominance of C18:1ω7c, C16:0 2-OH, C16:1ω7c, C17:1, followed by C15:0 2-OH and C14:0 2-OH in its fatty acid profile. Polar lipids
consisted of phosphatidylcholine, sphingoglycolipid, phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, an unknown phospholipid, and an unknown lipid. The DNA G+C content was 56.5 mol %. Based on phylogenetic analysis and distinctive phenotypic characteristics, strain 9574T is concluded to represent a novel species of the genus Sphingorhabdus, for which the name Sphingorhabdus pacificus sp. nov., is proposed. The type strain of the species is strain KMM 9574T (= NRIC 0922T = JCM 30177T). Keywords Sphingorhabdus pacificus sp. nov. · Marine · Sand sediments
Communicated by Erko Stackebrandt. The DDBJ/GenBank/EMBL accession number of the 16S rRNA gene sequence of strain KMM 9574T is AB936074.
Introduction
Electronic supplementary material The online version of this article (doi:10.1007/s00203-014-1033-8) contains supplementary material, which is available to authorized users.
The genus Sphingorhabdus was proposed by Jogler et al. (2013) to accommodate novel species Sphingorhabdus planktonica as the type species of the genus and to reclassify three related members of the genus Sphingopyxis, including Sphingopyxis flavimaris (Yoon and Oh 2005), Sphingopyxis litoris, and S. marina (Kim et al. 2008) as the species of the genus Sphingorhabdus, respectively. Three more novel species have been recently added to the genus, Sphingorhabdus arenilitoris, and two former Sphingopyxis species, S. rigui and S. wooponensis (Baik et al. 2013), being transferred to the genus Sphingorhabdus as Sphingorhabdus rigui and Sphingorhabdus wooponensis, respectively (Park et al. 2014, in press). It should be noted that most Sphingorhabdus bacteria have been isolated from marine coastal habitats (Yoon and Oh 2005; Kim et al. 2008; Park et al. in press), whereas S. planktonica was recovered from an oligotrophic freshwater lake (Jogler et al. 2013), and S. rigui and S. wooponensis from wetland
L. A. Romanenko (*) · V. V. Mikhailov G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch, Russian Academy of Sciences, Prospect 100 Let Vladivostoku, 159, 690022 Vladivostok, Russia e-mail: [email protected] N. Tanaka N
Data Loading...
