Detection and genetic characterization of a novel parvovirus (family Parvoviridae ) in barn owls ( Tyto alba ) in Hungar
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Detection and genetic characterization of a novel parvovirus (family Parvoviridae) in barn owls (Tyto alba) in Hungary Renáta Hargitai1 · Ákos Boros1 · Péter Pankovics1 · Róbert Mátics2,3 · Eda Altan4 · Eric Delwart4,5 · Gábor Reuter1 Received: 23 June 2020 / Accepted: 16 September 2020 © Springer-Verlag GmbH Austria, part of Springer Nature 2020
Abstract In this study, a novel parvovirus (gyb-MR02/2015/HUN, MT580795) was detected in barn owls (Tyto alba) and genetically characterized using viral metagenomics and PCR methods. The NS1 and VP1 proteins of gyb-MR02/2015/HUN share only 45.4% and 50.1% amino acid sequence identity, respectively, to the corresponding proteins of peafowl parvovirus 2 (MK988620), the closest relative. Out of 11 faecal specimens from owls (six from little owls, three from barn owls, and two from long-eared owls), two barn owl samples were positive for the novel parvovirus, which is distantly related to members of the recently established genus Chaphamaparvovirus in the subfamily Hamaparvovirinae. Systematic investigation is necessary to explore the diversity of parvoviruses. Parvoviruses are non-enveloped viruses with an approximately 4- to 6-kb-long single-stranded DNA genome that contains two major open reading frames (ORF). The nonstructural (NS) region encodes the enzymes used for replication, and the structural (VP) region encodes the capsid protein. Both ends of the genome contain hairpin-like untranslated genome termini [5]. The family Parvoviridae is divided into three subfamilies: Densovirinae, whose Handling Editor: Ana Cristina Bratanich. Nucleotide sequence data reported are available in the DDBJ/ EMBL/GenBank databases under the accession numbers MT580795 and MT580796. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00705-020-04862-6) contains supplementary material, which is available to authorized users. * Gábor Reuter [email protected] 1
Department of Medical Microbiology and Immunology, Medical School, University of Pécs, Szigeti út 12., Pecs 7624, Hungary
2
Department of Nature Conservation, University of Kaposvár, Kaposvár, Hungary
3
Hungarian Nature Research Society, Ajka, Hungary
4
Vitalant Research Institute, San Francisco, CA, USA
5
University of California, San Francisco, San Francisco, CA, USA
members infect invertebrates, and Parvovirinae and Hamaparvovirinae, whose members infect vertebrate hosts [5, 22]. The last of these is a new subfamily with five recognised genera: Brevihamaparvovirus, Chaphamaparvovirus, Hepanhamaparvovirus, Ichthamaparvovirus and Penstylhamaparvovirus [22]. The genus Chaphamaparvovirus includes a group of viruses that were previously called “chapparvoviruses” [20, 22]. The original name "chapparvovirus" comes from the first described hosts: chiropteran (Eidolon helvum), avian (turkey) and porcine [2, 21, 26]. Since then, there have been reports of further novel chaphamaparvoviruses in various countries from very diverse vertebrate hosts, including mam
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