Development and characterization of four moderate multiplex microsatellite panels in crucian carp ( Carassius auratus )
- PDF / 169,786 Bytes
- 3 Pages / 595.276 x 790.866 pts Page_size
- 101 Downloads / 165 Views
TECHNICAL NOTE
Development and characterization of four moderate multiplex microsatellite panels in crucian carp (Carassius auratus) Lei Cheng • Yan Zhang • Cui-Yun Lu Chao Li • Xiao-Wen Sun
•
Received: 3 January 2013 / Accepted: 22 March 2013 / Published online: 31 March 2013 Ó Springer Science+Business Media Dordrecht 2013
Abstract Sixteen microsatellite loci of Carassius auratus were successfully assigned into four multiplex panels in this study. These multiplex panels were characterized in 30 diploid C. auratus individuals from Songhua River. All loci were found to be polymorphic with the polymorphic information content ranging from 0.409 to 0.905. The number of alleles per locus varied from 3 to 16 and the observed heterozygosity (HO) ranged from 0.269 to 0.897. Three of these loci significantly deviated from Hardy– Weinberg expectations and no pairs of loci showed significant linkage disequilibrium. These multiplex microsatellite panels will be used to determine population structure of C. auratus, and detect hybridization between closely related carps. Keywords Carassius auratus Microsatellite Multiplex PCR Invasion
Introduction The Crucian carp (Carassius auratus) is a freshwater fish in the family Cyprinidae of order Cypriniformes, which is one of the most commonly kept food fish and ornamental fish (known as Goldfish), especially in East Asia (Chen and L. Cheng C.-Y. Lu C. Li X.-W. Sun (&) Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Harbin 150070, China e-mail: [email protected] L. Cheng e-mail: [email protected] Y. Zhang The Centre for Applied Aquatic Genomics, Chinese Academy of Fishery Sciences, Beijing 100141, China
Huang 1982; Hosoya 2002). Several subspecies and mitochondrial DNA lineages have been identified in the C. auratus, and these subspecies or lineages have been found to have high levels of regional specificity (Takada et al. 2010; Gao et al. 2012). However, artificial introductions have been identified in many regions, which might have broken down the local adaptation of C. auratus. Especially, several subspecies of C. auratus that are native to eastern Asian have been introduced into Europe, and now pose considerable ecological threats to closely related European carps (e.g. C. auratus and Cyprinus carpio) by competition and hybridization (Ha¨nfling et al. 2005; Jakovlic´ and Gui 2011; Kalous et al. 2013; Rylkova´ et al. 2012; Wouters et al. 2012). Microsatellite is a marker of choice in studies on migration and invasion, but we found that many SSR markers of C. auratus are poorly amplified or low polymorphic, such as markers identified by Guo and Gui (2008). Meanwhile, multiplexing SSR typing greatly decreases genotyping costs and increases throughput, but none of multiplex PCR assay panel is readily available for C. auratus. Thus, the primary purpose of this study was to develop moderate multiplex microsatellite panels in this species. The experiment was carried out essentially following the suggestions of Guichoux et al. (201
Data Loading...
