Transcriptome analysis of goldfish ( Carassius auratus ) in response to Gyrodactylus kobayashii infection
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IMMUNOLOGY AND HOST-PARASITE INTERACTIONS - ORIGINAL PAPER
Transcriptome analysis of goldfish (Carassius auratus) in response to Gyrodactylus kobayashii infection Shun Zhou 1,2,3 & Yongtao Liu 1,3 & Jing Dong 1,3 & Qiuhong Yang 1,3 & Ning Xu 1,3 & Yibin Yang 1,3 & Zemao Gu 2 & Xiaohui Ai 1,3 Received: 10 December 2019 / Accepted: 21 July 2020 # Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract Gyrodactylid monogeneans are widespread parasites of teleost fishes, and infection with these parasites results in high host morbidity and mortality in aquaculture. To comprehensively elucidate the immune mechanisms against Gyrodactylus kobayashii, the transcriptome profiles of goldfish (Carassius auratus) skin after challenge with G. kobayashii were first investigated using next-generation sequencing. Approximately 21 million clean reads per library were obtained, and the average percentage of these clean reads mapped to the reference genome was 82.25%. A total of 556 differentially expressed genes (DEGs), including 344 upregulated and 212 downregulated genes, were identified, and 380 DEGs were successfully annotated and assigned to 95 signaling pathways in Kyoto Encyclopedia of Genes and Genomes (KEGG). In addition, 14 pathways associated with immune response were identified mainly including mTOR signaling pathway, cytokine-cytokine receptor interaction, intestinal immune network for IgA production, toll-like receptor signaling pathway, and phagosome. Twelve genes were selected and validated using qRT-PCR. A similar trend of these genes between RNA-Seq and qRT-PCR was observed, indicating that RNA-Seq data was reliable. Besides, the ALP activity and NO content in serum were significantly higher in the infected goldfish compared with the non-infected goldfish. In summary, this study provides better understandings of immune defense mechanisms of goldfish against G. kobayashii, which will support future molecular research on gyrodactylids and facilitate the prevention and treatment of gyrodactylosis in aquaculture. Keywords Gyrodactylus kobayashii . Transcriptome . Skin . Carassius auratus . Immune response
Introduction Gyrodactylid monogeneans are widespread parasites that inhabit the skin, fins, and gills of various teleost fishes. Section Editor: Shokoofeh Shamsi Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00436-020-06827-9) contains supplementary material, which is available to authorized users. * Xiaohui Ai [email protected] 1
Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, No. 8 Wuda Park Road 1, Wuhan 430223, Hubei Province, China
2
Department of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural University, Wuhan 430223, China
3
Hu Bei Province Engineering and Technology Research Center of Aquatic Product Quality and Safety, Wuhan 430223, China
These helminths are viviparous and contain several generations of embryos in utero in the “Russian-doll” manner (Bakke et al. 2007). They are considered
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