Characterization of microsatellite loci in Sinilabeo rendahli and cross-amplification in four other Chinese cyprinid spe
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TECHNICAL NOTE
Characterization of microsatellite loci in Sinilabeo rendahli and cross-amplification in four other Chinese cyprinid species Ke Shao • Meihua Xiong • Nian Xu Bin Zhu • Fang Shi
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Received: 3 July 2012 / Accepted: 7 July 2012 / Published online: 17 July 2012 Ó Springer Science+Business Media B.V. 2012
Abstract Twenty-eight microsatellite loci were isolated in Sinilabeo rendahli, an endemic cyprinid fish in China. In a test on a population of 48 individuals, 15 primer pairs exhibited polymorphism with 2–12 alleles per locus, while the rest 13 were monomorphic. Observed and expected heterozygosities ranged from 0.104 to 0.915 and 0.195 to 0.865, respectively. Four loci showed significant deviation from Hardy–Weinberg equilibrium and two of them had evidence of null alleles. Twenty-six markers successfully cross-amplified in at least one of the four additional Chinese cyprinid species (Garra pingi pingi, Pseudogyrincheilus procheilus, Discogobio yunnanensis and Onychostoma sima) examined in this study. Keywords Sinilabeo rendahli Microsatellite loci Cross-species amplification
Sinilabeo rendahli (Kimura), an endemic cyprinid fish in China, used to have a wide range of distribution in the upper reaches of the Yangtze River and its main branches. It has great economic importance due to its edible and medicinal value. Unfortunately, habitat destruction, damming and overfishing have pushed the species to rarity in most of its historical range (Fu et al. 2003; He et al. 2011; Huang et al. 2011). A quantitative analysis on conservation
K. Shao M. Xiong N. Xu B. Zhu F. Shi (&) Key Laboratory of Ecological Impacts of Hydraulic-Projects and Restoration of Aquatic Ecosystem of Ministry of Water Resources, Institute of Hydroecology, Ministry of Water Resources and Chinese Academy of Sciences, Wuhan 430079, People’s Republic of China e-mail: [email protected]
prioritization of endemic fishes in the upper reaches of the Yangtze River listed S. rendahli as third-class protected species (Liu 2004). Since 2002, efforts have been taken to promote recovery of this endangered species, including artificial breeding and releasing and other conservation programs (He 2007). Current breeding programs of S. rendahli may result in inbreeding, genetic drift, and reduction of the effective population size, due to lack of effective genetic managements on this species. Therefore, it’s essential to gain a better understanding of its population genetic structure in order to perform effective conservation. However, in the past, most of the studies on S. rendahli focused on biology, nutritional components, and embryonic development (Xiong et al. 2007; Zhang et al. 2010), and little literature is available regarding its genetic characteristics. In this study, we established a partial genomic library for S. rendahli and developed a set of microsatellites that will be useful to investigate the genetic variability of S. rendahli for conservation purposes. Genomic DNA for constructing a microsatellite-enriched library wa
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