Dietary cadmium exposure causes elevation of blood ApoE with triglyceride level in mice
- PDF / 2,034,121 Bytes
- 14 Pages / 547.087 x 737.008 pts Page_size
- 37 Downloads / 180 Views
(0123456789().,-volV) (0123456789().,-volV)
Dietary cadmium exposure causes elevation of blood ApoE with triglyceride level in mice Haitao Liu . Yanwei Wang . Zhen Ren . Xingqi Ji . Frank Addai Peprah . Xiuwen Zhang . Shuya Dai . Yang Zhou . Jie Gu . Haifeng Shi
Received: 20 May 2020 / Accepted: 10 September 2020 Ó Springer Nature B.V. 2020
Abstract Cadmium (Cd) is a widespread toxic occupational and environmental pollutant, and its effect on lipid metabolism dysregulation has been observed. In this study, we utilized two-dimensional electrophoresis (2-DE) and mass spectrometry (MS) technologies to explore changes in the blood plasma proteins of mice exposed to Cd. From the 2-DE, 8 protein spots were screened in response to Cd exposure, and the identities of these proteins were revealed by MALDI-TOF MS. Western blotting was
Haitao Liu, Yanwei Wang, and Zhen Ren contributed equally to this work.
Electronic supplementary material The online version of this article (https://doi.org/10.1007/s10534-020-00247-z) contains supplementary material, which is available to authorized users. H. Liu H. Shi (&) School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212000, Jiangsu, China e-mail: [email protected] Y. Wang Z. Ren X. Ji F. A. Peprah X. Zhang S. Dai Y. Zhou J. Gu (&) H. Shi Institute of Life Sciences, Jiangsu University, Zhenjiang 212000, Jiangsu, China e-mail: [email protected] X. Zhang Changzhou No. 2 People’s Hospital, the Affiliated Hospital of Nanjing Medical University, Changzhou 213000, Jiangsu, China
applied to analyze the expression of the apolipoproteins in both plasma and liver, which were consistent with Cd-induced dyslipidemia of their composed lipid. Moreover, the Cd-induced apolipoprotein ApoE upregulation was due to inhibition of autopahgic flux in the Cd exposed mice. It was further observed from the mouse liver that Cd reduced the expression of the lipid uptake receptor low-density lipoprotein receptor (LDLR), which might be responsible for the coordinated elevation in blood triglycerides and abnormal apolipoproteins. This study may provide a new insight into the mechanism of Cd-induced dyslipidemia and the risk of cardiovascular diseases. Keywords Cadmium Proteomics Apolipoprotein Autophagy Abbreviations 2-DE Two-dimensional electrophoresis ABCA1 ATP-binding cassette transporter A1 AD Alzheimer’s disease Apcs Amyloid P-component precursor ApoA1p Apolipoprotein A-I precursor ApoA2 Apolipoprotein A-II ApoA4 Apolipoprotein A-IV ApoC3p Apolipoprotein C-III precursor ApoE Apolipoprotein E Cd Cadmium FFA Free (non-esterified) fatty acids HDL-C High density lipoprotein-cholesterol
123
Biometals
IEF LDL-C LDLR MTTP MS PZP PL RBP TC TG VLDL-C
Isoelectric focusing Low density lipoprotein-cholesterol Low-density lipoprotein receptor Microsomal triglyceride transfer protein Mass spectrometry Pregnancy zone protein Phospholipids Retinol-binding protein Total cholesterol Total triglycerides Very low density lipoprotein-cholesterol
Introduction Cadmium (Cd),
Data Loading...
