Dual release of VEGF and PDGF from emulsion electrospun bilayer scaffolds consisting of orthogonally aligned nanofibers
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esearch Letter
Dual release of VEGF and PDGF from emulsion electrospun bilayer scaffolds consisting of orthogonally aligned nanofibers for gastrointestinal tract regeneration Yu Zhou, Department of Mechanical Engineering, The University of Hong Kong, Pokfulam Road, Pokfulam, Hong Kong Qilong Zhao , Department of Mechanical Engineering, The University of Hong Kong, Pokfulam Road, Pokfulam, Hong Kong; Institute of Biomedical and Health Engineering, Shenzhen Institutes of Advanced Technology (SIAT), Chinese Academy of Sciences (CAS), Shenzhen, China Min Wang, Department of Mechanical Engineering, The University of Hong Kong, Pokfulam Road, Pokfulam, Hong Kong Address all correspondence to Q. Zhao at [email protected] and M. Wang at [email protected] (Received 22 May 2019; accepted 26 July 2019)
Abstract The regeneration of human tissues with complex anatomy such as gastrointestinal (GI) tract remains greatly challenging since it requires appropriate cell microenvironments with well-defined structural and biochemical cues. In this investigation, bilayer scaffolds consisting of different polymer nanofibers with orthogonal fiber orientations were prepared, in which vascular endothelial growth factor (VEGF) and platelet-derived growth factor (PDGF) were encapsulated separately. The bilayer scaffolds have similar architecture to the anatomy of the GI tract and can achieve dual releases of VEGF and PDGF in sequential and sustained manners, which hold promise as appropriate cell microenvironments for promoting the regeneration of the GI tract.
Introduction Partial resection of diseased tissues or organs is commonly employed in clinical treatment, while tissue loss may affect the quality of patients’ life or induce complications. Developing strategies for promoting tissue regeneration is hence of great significance. However, the regeneration of some tissues/organs with complicated anatomies such as gastrointestinal (GI) remains greatly challenging by using existing strategies. Since the GI tract contains abundant vascularized structures embedded in multiple tissue layers consisting of different cell types with specific cell organizations (typically one mucosal layer mainly consisting of epithelial cells and two smooth muscle layers with, respectively, longitudinal and parallel smooth muscle cell arrangements), desirable GI tract regeneration requires comprehensive efforts to first promote the reconstituting of vasculatures and subsequently assist the regeneration of different tissue layers, particularly smooth muscle layers with distinct anisotropies in cell arrangement.[1] In recent decades, tissue engineering scaffolds, as appropriate structural platform for supporting cell ingrowth and tissue remodeling, have attracted extensive attentions for assisting tissue regeneration.[2] Among different techniques for manufacturing tissue engineering scaffolds, electrospinning has shown significant merits in the ease and versatility to form nanofibrous structures resembling the architectures of native extracellular matrix, which have been wide
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