Effect of auxin and its synthetic analogues on the biomass production and biochemical composition of freshwater microalg
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Effect of auxin and its synthetic analogues on the biomass production and biochemical composition of freshwater microalga Ankistrodesmus falcatus CMSACR1001 Aravind K Vijay 1 & Syama Prabha 1 & Jubin Thomas 1 & Jeeva Susan Kurian 1 & Basil George 1 Received: 19 April 2020 / Revised and accepted: 25 August 2020 # Springer Nature B.V. 2020
Abstract Exogenous supplementation of phytohormones to promote the selective enrichment of biomolecules without hampering biomass production is regarded as a promising approach to improve the techno-economic feasibility of biofuels and other value-added products derived from microalgae. The present work outlines the influence of natural auxin, indole-3-acetic acid (IAA) and two synthetic analogues, 1-naphthalene acetic acid (NAA), and 2,4-dichlorophenoxyacetic acetic acid (2,4-D) on the growth pattern and biochemical productivity of the green alga Ankistrodesmus falcatus CMSACR1001. A maximum biomass production of 431 mg L−1 with a concomitant increase in the protein and carbohydrate content (20.2% and 49.6% DCW respectively) was achieved under supplementation of 10 μM IAA. Biomass productivity at 1 μM concentration was found to be comparable for both IAA and NAA (32.8 mg L−1 day−1 and 32.4 mg L−1 day−1). At higher supplemented concentrations 2,4-D induced a 30– 40% increase in carotenoid production and exhibited an increased lipid content of 41–47% DCW as compared with control (34.2% DCW). The current study also reports a substantial increase in the neutral lipid proportion as much as 73.9% and 79% under supplementation of NAA and 2,4-D respectively at 100 μM. The results indicate that the auxin and its analogues under the study could increase the biomass production and favourably modulate the biochemical composition of A. falcatus. Keywords Chlorophyta . Phytohormones . Biochemical stimulants . Ankistrodesmus falcatus . Lipid . Carbohydrates . Pigments
Introduction Elevated emissions of greenhouse gases in the atmosphere and limiting fossil reserves have encouraged researchers across the world to search for more environmentally acceptable and sustainable sources to suffice the energy requirements of the growing population. This prompted research on biomassbased energy generation or “biofuel” due to its potential sustainability and techno-economic feasibility. Nevertheless, not all biomass can be regarded as sustainable. For example, the first-generation biofuel feedstocks such as sugarcane and soya-oil are also food sources, which limit their applicability, whereas lignocellulosic biomass used in the secondElectronic supplementary material The online version of this article (https://doi.org/10.1007/s10811-020-02247-5) contains supplementary material, which is available to authorized users. * Basil George [email protected] 1
Department of Botany, CMS College Kottayam (Autonomous), Kottayam, Kerala 686001, India
generation biofuel may promote deforestation and resource depletion (Verma and Sharma 2016). Consequently, microalgal biomass is preferred over these candid
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