Effect of methyl jasmonate on the production of furanocoumarins in cell suspension cultures of Ruta graveolens L
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ORIGINAL ARTICLE
Effect of methyl jasmonate on the production of furanocoumarins in cell suspension cultures of Ruta graveolens L Talfan Anad Ahmad1 · Muthana Muhamad Al‑Mahdawe1 · Dhuha Sabah Nadir1 Received: 8 July 2020 / Accepted: 29 September 2020 © Springer Nature B.V. 2020
Abstract This research project aimed to study the eliciting effect of different concentrations of methyl jasmonate (MeJA) (0.0, 25, 50, 75 µM L −1) on the production of furanocoumarins (psoralen and bergapten) by harvested cells of batch cultures of Ruta graveolens L. grown in liquid Murashige and Skoog (MS) medium supplemented with 2.0 mg L−1 2,4-Dichlorophenoxy acetic acid (2,4-D) combined with 0.5 mg L −1 Kinetin (Kin), during three incubation periods (7, 14 and 21 days). The highest concentration of Psoralen recorded was 3.220 mg g−1 in the nutritional medium in a culture treated with 25 µM L −1 MeJA −1 after 21 days incubation. Bergapten reached its highest concentration of 8.223 mg g in the medium after 21 days incubation in a culture treated with 50 µM L−1 MeJA. Harvested cells showed a different picture, Psoralen highest concentration (3.495 mg g−1) was achieved after 14 days incubation in a culture treated with 75 µM L−1 MeJA, while the highest concentration of bergapten (7.110 mg g−1) was recorded after 21 days of incubation with 50 µM L −1 MeJA. These data support the potential use of cell suspension cultures as an alternative source for the field plants in the production of furanocoumarins from Ruta graveolens. Key Message Cell Suspension cultures are an excellent alternative source for field plants in the production of secondary metabolites from Ruta graveolens. Keywords Ruta graveolens L. · Furanocoumarins · Abiotic elicitors · Cell suspension cultures Abbreviations MS Murashige and Skoog Kin Kinetin 2,4-D 2,4- Dichlorophenoxy acetic acid MeJA Methyl jasmonate RPM Revolution per minute FLCC Fast Liquid Column Chromatography P Psoralen B Bergapten
Communicated by Christophe Hano. * Talfan Anad Ahmad [email protected] 1
College of Education for Pure Sciences, University of Diyala, Diyala, Iraq
Introduction Ruta graveolens L. belongs to the family Rutaceae, and has been used in the traditional medicine since the Greek and Roman times (Kannan and Babu 2012) due to its contents of many secondary metabolites, including essential oils, alkaloids, flavonoids and furanocoumarins (Fadlalla et al. 2011). In addition, it contains carotenoids, chlorophylls and several compounds with antimicrobial activities such as acridone. The plant is also characterised as a strong odoriferous herb (Wessner et al. 1999; Poutaraud et al. 2000). Plant cell, tissue and organ cultures have been used for more than three decades to produce secondary metabolites (Yue et al. 2016), as the fastest technology for a continuous in vitro production of large quantities of pharmaceuticals (Al-Sumaidai 2017), away from environmental constraints and without interferences from other compounds as might happen during isolation from the who
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