Ethylene Signaling Methods and Protocols
This volume provides a collection of protocols aimed toward the study of ethylene signaling in plants. Ethylene Signaling: Methods and Protocols is divided into three sections: ethylene biosynthesis, the signal transduction pathway, and the dive
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Brad M. Binder G. Eric Schaller Editors
Ethylene Signaling Methods and Protocols
Methods
in
Molecular Biology
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
Ethylene Signaling Methods and Protocols
Edited by
Brad M. Binder Department of Biochemistry & Cellular and Molecular Biology, University of Tennessee, Knoxville, TN, USA
G. Eric Schaller Department of Biological Sciences, Dartmouth College, Hanover, NH, USA
Editors Brad M. Binder Department of Biochemistry & Cellular and Molecular Biology University of Tennessee Knoxville, TN, USA
G. Eric Schaller Department of Biological Sciences Dartmouth College Hanover, NH, USA
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-6852-7 ISBN 978-1-4939-6854-1 (eBook) DOI 10.1007/978-1-4939-6854-1 Library of Congress Control Number: 2017931962 © Springer Science+Business Media LLC 2017 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. The publisher remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. Cover illustration: Apical hook of a dark-grown Arabidopsis seedling grown in the presence of ethylene. The seedling was visualized by collapsing multiple Z-stack images from a confocal, with red fluorescence arising from propidium iodide staining of the cell wall. Green fluorescence arises from an EIN3-GFP reporter, this appearing predominantly yellow due to overlap with red fluorescence. (photograph by Yan Zubo) Printed on acid-free paper This Humana Press imprint is published by Springer Nature The registered company is Springer Science+Business Media LLC The registered company address is: 233 Spring Street, New York, NY 10013, U.S.A.
Preface Ethylene was the first gaseous hormone discovered, and its discovery was prompted by the pronounced effects of “illuminating gas” on plant growth and
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