Faraday cage-type aptasensor for dual-mode detection of Vibrio parahaemolyticus
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ORIGINAL PAPER
Faraday cage-type aptasensor for dual-mode detection of Vibrio parahaemolyticus Wenting Wei 1 & Han Lin 1 & Huili Shao 1 & Tingting Hao 1 & Sui Wang 1 & Yufang Hu 1 & Zhiyong Guo 1
&
Xiurong Su 2
Received: 20 March 2020 / Accepted: 18 August 2020 # Springer-Verlag GmbH Austria, part of Springer Nature 2020
Abstract A Faraday cage-type aptasensor has been developed for dual-mode detection of a common bacterial pathogen Vibrio parahaemolyticus (VP) by electrochemiluminescence (ECL) and differential pulse voltammetry (DPV), using a multifunctionalized material Pb2+-Ru-MOF@Apt2 as signal unit. The recognition aptamer Apt2 recognizes VP; specifically, ruthenium-based metal organic framework (Ru-MOF) and lead ions (Pb2+) embedded produce an ECL signal at a working potential from 0 to 1.5 V and DPV signal from − 0.3 to − 0.8 V vs. Ag/AgCl. Since Ru-MOF is a two-dimensional conductive material signal unit overlapped onto the electrode surface to form a Faraday cage-type aptasensor. Thus, electrons could be easily exchanged between electrode and signal tags without being hindered by micron-size VP, resulting in a high detection sensitivity with a detection limit of 1.7 CFU mL−1. In addition, dual-mode detection was achieved, improving the accuracy and reliability of rapid field detection. Stability and selectivity were also satisfactory. The tests of real samples indicate that this Faraday cage-type aptasensor is suited for rapid detection of VP and analog pathogens and shows great potential in food safety.
Keywords Faraday cage-type aptasensor . Dual-mode detection . Vibrio parahaemolyticus . Ru-MOF two-dimensional material . Electrochemiluminescence . Differential pulse voltammetry
Introduction Accurate, sensitive, simple, and fast detection methods for Vibrio parahaemolyticus (VP) are of great importance [1, 2], which mainly include the following types: (1) traditional microbiological methods such as most probable number method,
Wenting Wei and Han Lin contributed equally to this work. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00604-020-04506-1) contains supplementary material, which is available to authorized users. * Zhiyong Guo [email protected] * Xiurong Su [email protected] 1
State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, State Key Laboratory Base of Novel Functional Materials and Preparation Science, School of Materials Science and Chemical Engineering, Ningbo University, Ningbo 315211, People’s Republic of China
2
State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, School of Marine Sciences, Ningbo University, Ningbo 315211, People’s Republic of China
colony counting method, and membrane filter method [3]. (2) Immunological methods including enzyme-linked immunosorbent assay (ELISA) [4, 5], fluorescent immunoassay (FIA) [6], chemiluminescence immunoassay (CLIA) [7], and other technologies based on immune reaction such
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