Fast Detection of DNA Damage Methods and Protocols
This volume presents a comprehensive collection of quick assays for the detection of nuclear and mitochondrial DNA damage and its effects in live and fixed cells and tissues, and in bacterial genomes. Although, such rapid techniques are in demand in the “
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Vladimir V. Didenko Editor
Fast Detection of DNA Damage Methods and Protocols
Methods
in
Molecular Biology
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
Fast Detection of DNA Damage Methods and Protocols
Edited by
Vladimir V. Didenko Department of Neurosurgery and Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, Texas, USA
Editor Vladimir V. Didenko Department of Neurosurgery and Department of Molecular and Cellular Biology Baylor College of Medicine Houston, TX, USA
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-7185-5 ISBN 978-1-4939-7187-9 (eBook) DOI 10.1007/978-1-4939-7187-9 Library of Congress Control Number: 2017944623 © Springer Science+Business Media LLC 2017 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. The publisher remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. Printed on acid-free paper This Humana Press imprint is published by Springer Nature The registered company is Springer Science+Business Media LLC The registered company address is: 233 Spring Street, New York, NY 10013, U.S.A.
Preface Research in DNA damage detection is a fast-paced field of study. The continuous requirement in this field for ever faster DNA damage assays is driven by the rapidly increasing numbers of samples to be analyzed and by the intensified competition between various scientific groups. The faster assays make possible rapid high-volume and machine-based assessments. Such capability is in demand in drug design, high-throughput industry, environmental studies, and in molecular and clinical pathology research. It enables a pathology laboratory to perform rapid evaluations of DNA damage in either fixed archived tissues or unfixed clinical samples. The ubiquitous nature of DNA damage and its ou
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