Fecal microbiota of lambs fed purple prairie clover ( Dalea purpurea Vent.) and alfalfa ( Medicago sativa )
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ORIGINAL PAPER
Fecal microbiota of lambs fed purple prairie clover (Dalea purpurea Vent.) and alfalfa (Medicago sativa) Qianqian Huang1,2,3 · Devin B. Holman2 · Trevor Alexander2 · Tianming Hu3 · Long Jin2 · Zhongjun Xu2 · Tim A. McAllister2 · Surya Acharya2 · Guoqi Zhao1 · Yuxi Wang2
Received: 19 January 2017 / Revised: 30 June 2017 / Accepted: 22 August 2017 © Springer-Verlag GmbH Germany 2017
Abstract The present study assessed the effect of purple prairie clover (PPC) and PPC condensed tannins (CT) on the fecal microbiota of lambs using high-throughput 16S rRNA gene pyrosequencing. A total of 18 individual lambs were randomly divided into three groups and fed either green chop alfalfa (Alf), a 40:60 (DM basis; Mix) mixture of Alf and PPC, or Mix supplemented with polyethylene glycol (Mix-P) for 18 days. Fecal samples were collected on days 13 through 18 using digital rectal retrieval. The DNA of fecal samples was extracted and the microbial 16S rRNA gene amplicons were sequenced using 454 pyrosequencing. Regardless of diet, the bacterial community was dominated by Firmicutes and Bacteroidetes with many sequences unclassified at the genus level. Forage type and CT had no effect on the fecal microbial composition at the phylum level or on α-diversity. Compared to the Alf diet, the Mix diet reduced the relative abundance of Akkermansia (P = 0.03) and Asteroleplasma (P = 0.05). Fecal microbial populations in Alf and Mix-P clustered separately from each other when assessed using unweighted UniFrac (P
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