First report of southern blight on kale ( Brassica oleracea var. acephala ) caused by Athelia rolfsii in Turkey
- PDF / 152,920 Bytes
- 2 Pages / 595.276 x 790.866 pts Page_size
- 99 Downloads / 169 Views
DISEASE NOTE
First report of southern blight on kale (Brassica oleracea var. acephala) caused by Athelia rolfsii in Turkey Muharrem Türkkan 1
&
Göksel Özer 2 & Halil İbrahim Benli 1 & İsmail Erper 3,4
Received: 27 December 2019 / Accepted: 1 April 2020 # Società Italiana di Patologia Vegetale (S.I.Pa.V.) 2020
Keywords Brassica oleracea var. acephala . Athelia rolfsii . rDNA-ITS region
Kale (Brassica oleracea L. var. acephala) has been commonly cultivated in the Black Sea Region, Turkey. In 2017, wilting, subsequent death symptoms, and brown sclerotia and a mass of white cottony mycelium at the stem bases were observed on kale plants grown in three surveyed fields located in Ordu province, with a disease incidence of 12.5%. To isolate the pathogen, small sections were excised from the symptomatic tissues, plated on Potato Dextrose Agar (PDA) amended with antibiotics after a superficially-disinfestation and incubated at 25 °C in the dark for 10 days. The fungal colonies with white mycelium and large numbers of round creamy sclerotia were obtained on PDA. The septate hyphae with clamp connections were determined by examining under a light microscope. After three weeks, numerous small, brown, round sclerotia, 1.88 ± 0.03– 2.02 ± 0.04 mm in diameter, (n = 78), were developed on the media. These morphological features were typical of those described for Athelia rolfsii (Curzi) C.C. Tu & Kimbr. (anamorph Sclerotium rolfsii Sacc.) (Mordue 1974). The internal transcribed spacer (ITS) of rDNA region of a representative isolate (Kale078) was amplified using primers ITS1/ITS4 (White et al. 1990) and
* Muharrem Türkkan [email protected]; [email protected] 1
Plant Protection Department, Agriculture Faculty, Ordu University, 52200 Ordu, Turkey
2
Plant Protection Department, Agriculture and Natural Sciences Faculty, Bolu Abant Izzet Baysal University, 14030 Bolu, Turkey
3
Plant Protection Department, Agriculture Faculty, Ondokuz Mayis University, Atakum, 55139 Samsun, Turkey
4
Plant Protection Department, Agriculture Faculty, Kyrgyz Turkish Manas University, 720044 Bishkek, Kyrgyzstan
sequenced (GenBank Accession No. MN872304). BLASTn analysis of the 687 bp amplified fragment showed 100% similarity with the sequences of A. rolfsii isolates 63–76 and CBS132553 (AB042626 and JX566993, respectively). Aggressiveness of the isolate was tested on six healthy seedlings of kale cv. Arzuman at five-six true leaves stage. The base of stem was inoculated by 5-mm mycelial plugs from colonies grown on PDA while controls were inoculated with sterile mycelial plugs, and all seedlings were incubated at 25 °C with a 12-h photoperiod. The experiment was repeated twice. After 10 days, wilting and collapse of entire seedlings were observed on inoculated seedlings while controls remained symptomless. The fungus was successfully re-isolated and identified as described above to fulfill Koch’s postulates. To our knowledge, this is the first report of southern blight on kale caused by Athelia rolfsii in Turkey (Farr and Ros
Data Loading...
