Plant Membrane Proteomics Methods and Protocols
This volume provides comprehensive and detailed protocols that discuss proteomic techniques, plant endosomes, and isolation of organelles and subcellular fractions. The chapters in this book explore numerous plant species and cover topics, such as i
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Hans-Peter Mock Andrea Matros Katja Witzel Editors
Plant Membrane Proteomics Methods and Protocols
METHODS
IN
MOLECULAR BIOLOGY
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
Plant Membrane Proteomics Methods and Protocols
Edited by
Hans-Peter Mock and Andrea Matros Leibniz Institute of Plant Genetics and Crop Plant Research, Gatersleben, Germany
Katja Witzel Leibniz Institute of Vegetable and Ornamental Crops, Großbeeren, Germany
Editors Hans-Peter Mock Leibniz Institute of Plant Genetics and Crop Plant Research Gatersleben, Germany
Andrea Matros Leibniz Institute of Plant Genetics and Crop Plant Research Gatersleben, Germany
Katja Witzel Leibniz Institute of Vegetable and Ornamental Crops Großbeeren, Germany
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-7409-2 ISBN 978-1-4939-7411-5 (eBook) https://doi.org/10.1007/978-1-4939-7411-5 Library of Congress Control Number: 2017954368 © Springer Science+Business Media LLC 2018 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. The publisher remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. Printed on acid-free paper This Humana Press imprint is published by Springer Nature The registered company is Springer Science+Business Media LLC The registered company address is: 233 Spring Street, New York, NY 10013, U.S.A.
Preface Proteomics has found its way into many plant science laboratories and is complementing other large-scale “omics” approaches. Separation of complex protein samples has been achieved using 2-D gel electrophoresis long before suitable mass spectrometrical techniques were available for routine analysis. Only with the progress of nucleotide sequencing techniques the necessary databases were established to allow proper identification of relevant protein candidates. The ad
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