Phospho-Proteomics Methods and Protocols
This second edition provides up-to-date protocols focusing on techniques for the specific enrichment of phosphopeptides and phosphoproteins. Phospho-Proteomics Methods and Protocols, Second Edition guides readers through different labeling strategies for
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Louise von Stechow Editor
PhosphoProteomics Methods and Protocols Second Edition
METHODS
IN
MOLECULAR BIOLOGY
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
Phospho-Proteomics Methods and Protocols
Edited by
Louise von Stechow Proteomics Program, Novo Nordisk Foundation Center for Protein Research, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark
Editor Louise von Stechow Proteomics Program, Novo Nordisk Foundation Center for Protein Research Faculty of Health and Medical Sciences, University of Copenhagen Copenhagen, Denmark
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-3048-7 ISBN 978-1-4939-3049-4 (eBook) DOI 10.1007/978-1-4939-3049-4 Library of Congress Control Number: 2015945106 Springer New York Heidelberg Dordrecht London © Humana Press, a part of Springer Science+Business Media, LLC 2009 © Springer Science+Business Media New York 2016 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. Printed on acid-free paper Humana Press is a brand of Springer Springer Science+Business Media LLC New York is part of Springer Science+Business Media (www.springer.com)
Preface Phosphorylation of proteins is a crucial mechanism for regulating cell signaling processes and critically involved in essentially all physiological and pathological processes. Phosphorylation-mediated signaling processes are often deregulated in diseases, such as cancer. Monitoring protein phosphorylation in a given cell or tissue is vital for a better understanding of biological mechanisms underlying physiological and pathological processes. Phosphoproteomics: Methods and Protocols presents protocols and strategies for identification, evaluation, and quantitation of protein phosphorylation on Tyr, Ser, and Thr residues. This volume summarizes protocols focusing on techniques for the
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