Proteomics Methods and Protocols

This volume aims to provide protocols on a wide range of biochemical methods, analytical approaches, and bioinformatics tools developed to analyze the proteome. Written in the highly successful Methods in Molecular Biology series format, chapters include

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Lucio Comai · Jonathan E. Katz Parag Mallick Editors

Proteomics Methods and Protocols

Methods

in

Molecular Biology

Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK

For further volumes: http://www.springer.com/series/7651

Proteomics Methods and Protocols

Edited by

Lucio Comai Keck School of Medicine, University of Southern California, Los Angeles, CA, USA

Jonathan E. Katz Keck School of Medicine, University of Southern California, Los Angeles, CA, USA

Parag Mallick Stanford University, Palo Alto, CA, USA

Editors Lucio Comai Keck School of Medicine University of Southern California Los Angeles, CA, USA

Jonathan E. Katz Keck School of Medicine University of Southern California Los Angeles, CA, USA

Parag Mallick Stanford University Palo Alto, CA, USA

ISSN 1064-3745     ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-6745-2    ISBN 978-1-4939-6747-6 (eBook) DOI 10.1007/978-1-4939-6747-6 Library of Congress Control Number: 2017930810 © Springer Science+Business Media LLC 2017 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. Printed on acid-free paper This Humana Press imprint is published by Springer Nature The registered company is Springer Science+Business Media LLC The registered company address is: 233 Spring Street, New York, NY 10013, U.S.A.

Preface In the catalog of biochemical techniques, proteomics has barely reached its adolescent stage, and a very immature adolescent at that. Like any teen, potential still overshadows realized accomplishment, but the future is still bright with potential. This particular adolescent has shown quite a level of promise. Indeed, for a number of tasks, proteomics is fully proficient— determining the identity of a small number of proteins, providing absolute quantitation of a similar number of proteins. For others, it is still testing its limits: How many proteins? How many orders of magnitude of sensitivity? And we be