Shotgun Proteomics Methods and Protocols

Shotgun Proteomics: Methods and Protocols serves as a vital collection of protocols through which thousands of proteins can be simultaneously identified, quantified, and characterized in a high throughput manner.  Beginning with the history of proteo

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Daniel Martins-de-Souza Editor

Shotgun Proteomics Methods and Protocols

METHODS

IN

M O L E C U L A R B I O LO G Y

Series Editor John M. Walker School of Life Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK

For further volumes: http://www.springer.com/series/7651

Shotgun Proteomics Methods and Protocols

Edited by

Daniel Martins-de-Souza Laboratory of Neuroproteomics, Department of Biochemistry, Institute of Biology, State University of Campinas (UNICAMP), Campinas, Brazil; Max Planck Institute of Psychiatry, Munich, Germany; Research Group of Proteomics, Department of Psychiatry and Psychotherapy, Ludwig-Maximilians-University of Munich, Munich, Germany; Laboratory of Neurosciences (LIM-27), Institute of Psychiatry, University of Sao Paulo, Sao Paulo, Brazil

Editor Daniel Martins-de-Souza Laboratory of Neuroproteomics Department of Biochemistry Institute of Biology, State University of Campinas (UNICAMP) Campinas, Brazil Max Planck Institute of Psychiatry Munich, Germany Research Group of Proteomics Department of Psychiatry and Psychotherapy Ludwig-Maximilians-University of Munich, Munich, Germany Laboratory of Neurosciences (LIM-27) Institute of Psychiatry University of Sao Paulo Sao Paulo, Brazil

Additional material to this book can be downloaded from http://extras.springer.com ISSN 1064-3745 ISSN 1940-6029 (electronic) ISBN 978-1-4939-0684-0 ISBN 978-1-4939-0685-7 (eBook) DOI 10.1007/978-1-4939-0685-7 Springer New York Heidelberg Dordrecht London Library of Congress Control Number: 2014936840 © Springer Science+Business Media New York 2014 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. Exempted from this legal reservation are brief excerpts in connection with reviews or scholarly analysis or material supplied specifically for the purpose of being entered and executed on a computer system, for exclusive use by the purchaser of the work. Duplication of this publication or parts thereof is permitted only under the provisions of the Copyright Law of the Publisher’s location, in its current version, and permission for use must always be obtained from Springer. Permissions for use may be obtained through RightsLink at the Copyright Clearance Center. Violations are liable to prosecution under the respective Copyright Law. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. While the advice and information in this book are believed to be true and