Genetic diversity analysis of phenotypic character and SRAP molecular markers in 45 tree peony cultivars
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ORIGINAL ARTICLE
Genetic diversity analysis of phenotypic character and SRAP molecular markers in 45 tree peony cultivars Shumin Gao1 · Richen Cong2 · Lan Gao3 · Yuanyuan Zhu1,2 · Yuan Meng3 · Yan Zhou1,2 Received: 26 December 2018 / Revised: 2 February 2020 / Accepted: 27 February 2020 / Published online: 21 May 2020 © Botanical Society of Sao Paulo 2020
Abstract Tree peony (Paeonia suffruticosa Andrews) is a famous ornamental flowering species and favorite of people all over the world. In the present study, the genetic diversity of 12 phenotypic characters and 11 sequence-related amplified polymorphism (SRAP) markers was analyzed using the SPSS software in 45 tree peony cultivars. (1) Morphological-clustering results showed that 45 cultivars were classified into three groups: Group 1 includes 21 Central Plains cultivars, group 2 includes four Japanese cultivars and six Central Plains cultivars, and group 3 includes five Paeonia rockii T. Hong et J. J. Li cultivars, 8 Central Plains cultivars and one Japanese cultivar. (2) SRAP molecular markers analysis results showed that the 45 tree peony cultivars were divided into three groups, and five P. rockii cultivars clustered together as a group. ‘Dou Lv’ was independent group. Thirty-four Central Plains cultivars and five Japanese introduced cultivars clustered into a class were divided into four subgroups. The origin of the initial species of Japanese cultivars was Central Plains in China. (3) The analysis results by two classification methods evidently indicates that the SPAR molecular marker clustering presented the more exact and reliable genetic relationship, and both the external morphological characteristics and molecular marker clustering analysis combined was benefit for clear the germplasm resources diversity of tree peony cultivars and breeding improved varieties. SRAP molecular markers analysis results provided more details about tree peony genetic diversity and phyletic evolution than morphological-clustering results. Keywords Clustering analysis · Correlation coefficient · Germplasm resources diversity · Phyletic evolution · Sequencerelated amplified polymorphism (SRAP)
1 Introduction
Electronic supplementary material The online version of this article (https://doi.org/10.1007/s40415-020-00596-6) contains supplementary material, which is available to authorized users. * Yan Zhou [email protected] 1
College of Biological Sciences and Biotechnology, Beijing Forestry University, National Engineering Laboratory for Tree Breeding, No. 35 Tsinghua East Road, Haidian District, Beijing 100083, China
2
Beijing Key Laboratory of Greening Plants Breeding, Beijing Institute of Landscape Architecture, No. 7 Huajiadi, Chaoyang District, Beijing 100102, China
3
Beijing Jingshan Park, No. 44 Jingshan Hill Street, Xicheng District, Beijing 100009, China
The tree peony (Paeonia suffruticosa Andrews) is a famous ornamental flowering species with a rich ethnobotanical history in Chinese culture and has recently been used as an evolutionary model of pl
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