Whole Genome Amplification Methods and Protocols
This volume mirrors the holistic feature of whole genome amplification (WGA) technology by combining reviews, detailed basic methods, and advanced sample workflows. The first part of the book covers an overview of the development of WGA techniques through
- PDF / 5,637,244 Bytes
- 286 Pages / 504.63 x 737.01 pts Page_size
- 79 Downloads / 191 Views
Thomas Kroneis Editor
Whole Genome Amplification Methods and Protocols
METHODS
IN
MOLECULAR BIOLOGY
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
Whole Genome Amplification Methods and Protocols
Edited by
Thomas Kroneis Institute of Cell Biology, Histology & Embryology, Medical University of Graz, Graz, Austria
Editor Thomas Kroneis Institute of Cell Biology Histology & Embryology Medical University of Graz Graz, Austria
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-2989-4 ISBN 978-1-4939-2990-0 (eBook) DOI 10.1007/978-1-4939-2990-0 Library of Congress Control Number: 2015945590 Springer New York Heidelberg Dordrecht London © Springer Science+Business Media New York 2015 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. Cover image: Cells captured on an in vivo cell enrichment device (CANCER03, GILUPI GmbH, Potsdam, Germany) as seen by scanning electron microscopy [rectangular: 50x; small circular: 500x; big circular: original 200x, artificially enlarged)] The images show HT-29 cell line cells (human colorectal adenocarcinoma) that were isolated from a cell suspension using the new version of an already clinically used medical device (GILUPI CellCollector® CANCER01) that allows isolating circulating tumor cells from peripheral blood of cancer patients. This new device will allow pre-enrichment of CTCs directly from patient blood by a “catch and release” procedure resulting in single cell suspensions that are highly enriched for CTCs. For assessing the performance and applicability of this new method we subjected single HT-29 cells isolated by means of the new device to single-cell whole genome amplification (WGA, chapter 7). Resulting WGA products were assessed by DNA smear analysis and quality control-PCR (chapter 9). Samples meeting the criteria for high quality DNA were forwarded
Data Loading...
