Genome-wide identification of fasciclin-like arabinogalactan proteins in jute and their expression pattern during fiber
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ORIGINAL ARTICLE
Genome-wide identification of fasciclin-like arabinogalactan proteins in jute and their expression pattern during fiber formation Md. Sabbir Hossain1 · Borhan Ahmed1,2 · Md. Wali Ullah1 · Nasima Aktar1 · Md. Samiul Haque1,2 · Md. Shahidul Islam1,2 Received: 12 June 2020 / Accepted: 23 September 2020 © Springer Nature B.V. 2020
Abstract Fasciclin-like arabinogalactan proteins (FLAs), a class of arabinogalactan proteins (AGPs) are involved in plant growth and development via cell communication and adhesion. FLAs were also associated with fiber and wood formation in plants but no information is available about the roles of FLA proteins during fibre development of jute. Here, we performed molecular characterization, evolutionary relationship and expression profiling of FLAs proteins in jute (Corchorus olitorius). In total, nineteen CoFLA genes have been identified in jute genome, which were divided into four classes like FLAs of other species based on protein structure and similarity. All CoFLAs have N-terminal signal peptide and one or two FAS domain while two FLAs lack well defined AGP region and eight FLAs were devoid of C-terminal glycosylphosphatidylinositol (GPI) anchor. Expression analysis of different regions of jute stem suggested their involvement in different fiber development stages. Four genes CoFLA 11, 12, 20, and 23 were highly or predominately expressed in fiber containing bark tissues while the expression levels of six CoFLA genes 02, 03, 04, 06, 14 and 19 were comparatively higher in stick. Higher transcripts levels of CoFLA 12 and 20 in the middle bark tissues suggest their involvement in fiber elongation. In contrast, the CoFLA 11 and 23 were more expressed in bottom bark tissues suggesting their potential involvement in secondary cell wall synthesis. Our study can serve as solid foundation for further functional exploration of FLAs and in future breeding program of jute aiming fiber improvement. Keywords Fasciclin domain · Corchorus olitorius · Fiber elongation · Secondary cell wall · RT-qPCR
Introduction Plant cell wall, mainly composed of polysaccharides, crosslinking glycans and proteins, plays an important role during plant development processes and adaptation to various environmental conditions through interaction plasma membrane [1, 2]. Proteins such as enzymes, expansins, cell wall-associated kinases and hydroxyproline (Hyp)-rich glycoproteins comprise a small portion of primary cell wall Electronic supplementary material The online version of this article (https://doi.org/10.1007/s11033-020-05858-w) contains supplementary material, which is available to authorized users. * Md. Shahidul Islam [email protected]; [email protected] 1
Basic and Applied Research on Jute Project, Bangladesh Jute Research Institute, Dhaka, Bangladesh
Bangladesh Jute Research Institute, Dhaka, Bangladesh
2
(less than 10% of dry weight) with potential role in cell wall and plasma membrane interactions [1, 3]. Arabinogalactan proteins (AGP) belonging to Hyp-rich glycoprotein
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